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| Status |
Public on Dec 01, 2012 |
| Title |
Deep sequencing analysis of the murine transcriptome response to different virulent parenthood B. melitensis infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we employed the Illumina Genome Analyzer platform to perform a Digital Gene Expression (DGE) analysis of the peritoneal macrophages genome-wide transcriptome response to B.melitensis infection. Common changes in gene expression were observed among Brucella app. infected macrophages suggesting similar strategies were employed for their survival and replication, inducing anti-inflammatory and anti-apoptotic. A total of 1019 differentially expressed (DE) transcripts were identified in the macrophages 4 hours after differ virluent B.melitensis infection, especially genes participated lysosome pathway and MAPK pathway. Our findings demonstrate previously unrecognized changes in gene transcription that are associated with B.melitensis infection in the macrophages, and many significant pathways (cascades) identified in the study clearly merit further investigation. Our data provide new clues to understand the molecular attenuation mechanism of M5-90 and a firm foundation for reducing vaccine residual virulence, enhancing vaccine efficacy.
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| Overall design |
Examination oftwo peritoneal macrophages infected brucella and one blank control.
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| |
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| Contributor(s) |
Wang FK, Bu ZG |
| Citation(s) |
22216095 |
| Submission date |
Dec 28, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Fangkun Wang |
| E-mail(s) |
wangfangkun1980@163.com
|
| Organization name |
Chinese Academy of Agricultural Sciences
|
| Department |
Harbin Veterinary Research Institute
|
| Lab |
Zoonosis Laboratory of the Ministry of Agriculture
|
| Street address |
427 Maduan Street
|
| City |
Harbin |
| ZIP/Postal code |
150001 |
| Country |
China |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (3) |
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| Relations |
| SRA |
SRP005103 |
| BioProject |
PRJNA136913 |
