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Series GSE26363 Query DataSets for GSE26363
Status Public on Dec 31, 2010
Title Hepatic gene expression profile in hydrogen water administrated SD rat
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The effects of the administration of molecular hydrogen-saturated drinking water (hydrogen water) on hepatic gene expression were investigated in rats. Using DNA microarrays, 548 upregulated and 695 downregulated genes were detected in the liver after a 4-week administration of hydrogen water. Gene Ontology analysis revealed that genes for oxidoreduction-related proteins, including hydroxymethylglutaryl CoA reductase, were significantly enriched in the upregulated genes.
Overall design 4-week-old male Sprague-Dawley rats were acclimatized for 4 days in a room maintained at 24 ± 3°C with a relative humidity of 55 ± 15% with a 12-h light-dark cycle (lights on at 7:00, off at 19:00) and were fed ad libitum on a commercial diet (MF, Oriental Yeast Co., Yokohama, Japan). After acclimatization, the rats were divided into two groups (n = 8 per group) with similar mean body weights. During a period of 4 weeks, one group (control group) was supplied with sterilized distilled water and the other (HW group) with hydrogen water produced by MiZ Co. Ltd. (Fujisawa, Japan), which contains 0.7 mM dissolved hydrogen (pH 7.4). All the animals drank water ad libitum. After 4 weeks, each rat was anesthetized with diethyl ether and blood samples were collected from the abdominal aorta. The liver was then excised and analyzed the effect of hydrogen water extract administration on hepatic gene expression.
Contributor(s) Nakai Y, Sato B, Ushiama S, Okada S, Abe K, Arai S
Citation(s) 21512236
Submission date Dec 30, 2010
Last update date Jul 31, 2017
Contact name Yuji Nakai
Organization name Hirosaki University
Department Institute of Regional Innovation
Lab Section of Food Sciences
Street address 2-1-1, Yanagawa
City Aomori
State/province Aomori
ZIP/Postal code 038-0012
Country Japan
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (8)
GSM647246 Liver, Control (sterilized distilled water administrated for 4 weeks) #1
GSM647247 Liver, Control (sterilized distilled water administrated for 4 weeks) #2
GSM647248 Liver, Control (sterilized distilled water administrated for 4 weeks) #3
BioProject PRJNA136881

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26363_RAW.tar 19.9 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table

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