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| Status |
Public on Jan 15, 2011 |
| Title |
Gene expression under normoxia and hypoxia in mouse embryonic fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mouse and human somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by the transduction of four transcription factors, Oct 3/4, Sox2, Klf4, and c-Myc. Conducting reprogramming in hypoxic conditions was shown to result in improved efficiency for both mouse and human cells. To examine the effect of hypoxic cultivation, we performed microarray analyses using mouse embryonic fibroblasts cultivated under normoxia and hypoxia.
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| Overall design |
Mouse embryonic fibroblasts and four factor-transduced reprogramming fibroblasts were grown under normoxia and hypoxia. These cells were used for microarray analyses.
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| Contributor(s) |
Yoshida Y, Takahashi K, Okita K, Ichisaka T, Yamanaka S |
| Citation(s) |
19716359 |
| Submission date |
Jan 07, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA136489 |
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