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Series GSE26560 Query DataSets for GSE26560
Status Public on Aug 04, 2011
Title Expression data from Tcf1-expressing Thy1+CD25+ T lineage cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary We used microarrays to perform a global gene expression analysis in Tcf1-expressing Thy1+CD25+ T lineage cells that develop on OP9 stroma in the absence of Notch1 signals. We compare this to the starting population, LMPP progenitors, and to control expressing T lineage cells that developed on OP9 stroma expressing Notch ligand DL4. The overall goal of this study was to determine if Tcf1 initiates T lineage specification in lymphoid progenitors. We found that Tcf1 was sufficient to upregulate many T lineage genes as compared to control expressing progenitors on OP9-DL4.
Abstract of manuscript: The thymus imposes the T cell fate on incoming multipotent progenitors, but the molecular mechanisms are poorly understood. We show that transcription factor Tcf1 initiates T-lineage-specific gene expression. Tcf1 is downstream of Notch1 signaling and expressed in early T-cell progenitors. Progenitors deficient for Tcf1 are unable to initiate normal T-lineage specification. Conversely, ectopic expression of Tcf1 in hematopoietic progenitors is sufficient to induce expression of T-lineage specific genes in vitro. Thus, our study identifies Tcf1 as critically involved in the establishment T cell identity.
 
Overall design Wiltype LMPPs were isolated by a FACSAria cell sorter and retrovirally transduced with a Tcf1-containing (Tcf1-VEX) or control vector (VEX) retrovirus. Tcf1-expressing cells and control-vector expressing cells were then seeded on OP9 stroma or OP9 stroma expressing Notch ligand DL4, respectively. On day 10, Thy1+CD25+ T lineage cells were sorted from Tcf1-expressing cells on OP9 stroma and compared to sorted LMPPs and Thy1+CD25+ T lineage cells that developed from control-vector expressing cells on OP9-DL4.
 
Contributor(s) Brittany W, Anthony C, Alex C, Yumi Y, Avinash B
Citation(s) 21814277
Submission date Jan 11, 2011
Last update date Mar 04, 2019
Contact name Avinash Bhandoola
E-mail(s) bhandooa@mail.med.upenn.edu
Phone 2155730274
Organization name University of Pennsylvania
Department Pathology
Lab 266 John Morgan
Street address 3620 Hamilton Walk
City Philadelphia
State/province Philadelphia
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (4)
GSM652995 Pooled wiltype LMPPs (Lineage marker-Sca+kit+Flt3high)
GSM652996 Tcf1-expressing Thy1+CD25+ Cells Biological replicate #1
GSM652997 Tcf1-expressing Thy1+CD25+ Cells Biological replicate #2
Relations
BioProject PRJNA136225

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26560_RAW.tar 17.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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