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| Status |
Public on Dec 20, 2011 |
| Title |
Expression data from Control and Yorkie overexpression pupal eye discs |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
To probe mechanistic determinants of Yki function in mitochondrial biogenesis, we conducted a genome-wide microarray experiment, and specifically compared expression patterns of genes from control (GMR gal4) and Yorkie over-expressing (GMR gal4; UAS yki) pupal eye discs.
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| Overall design |
The above mentioned genotypes were grown at 29 deg C and at about 40 hrs after pupariation, the pupal eye discs were dissected. RNA was extracted from the pupal eye discs, purified and used to generate microarray probes that were hybridized to the Drosophila genome 2 arrays (Affymetrix). The Gene Chip Operating system (Affymetrix) and dCHIP program (Harvard University) were used to generate pairwise comparisons between the transcription profiles of control and Yorkie over-expressing discs.
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| Contributor(s) |
Rao SG, Banerjee U |
| Citation missing |
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| Submission date |
Jan 19, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shubha Gururaja Rao |
| E-mail(s) |
sgrao@ucla.edu
|
| Organization name |
UCLA
|
| Department |
MCDB
|
| Lab |
Banerjee Lab
|
| Street address |
5032 Terasaki Life Sciences Building, 621 Charles E. Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90034 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (4)
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| GSM657200 |
Control discs, biological rep1 |
| GSM657201 |
Control discs, biological rep2 |
| GSM657202 |
Yorkie overexpressing discs, biological rep1 |
| GSM657203 |
Yorkie overexpressing discs, biological rep2 |
|
| Relations |
| BioProject |
PRJNA136153 |
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