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| Status |
Public on Mar 01, 2011 |
| Title |
Gene expression analysis of 12 B-cell Chronic Lymphocytic Leukemia samples and 5 CD19+ control samples |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Elevated levels of microRNA miR-155 represent a candidate pathogenic factor in chronic B-lymphocytic leukemia (B-CLL). In this study, we present evidence that MYB (v-myb myeloblastosis viral oncogene homolog) is overexpressed in a subset of B-CLL patients. MYB physically associates with the promoter of MIR155 host gene (MIR155HG, also known as BIC, B-cell integration cluster) and stimulates its transcription. This coincides with the hypermethylated histone H3K4 residue and spread hyperacetylation of H3K9 at MIR155HG promoter. Our data provide evidence of oncogenic activities of MYB in B-CLL that include its stimulatory role in MIR155HG transcription.
We have studied differentially expressed genes in the whole genome and also in the preselected groups of MYB target genes and miR-155 microRNA predicted targets.
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| Overall design |
This study was intended to investigate differentially expressed genes mRNA in 12 B-CLL patient peripheral blood samples in comparison with 5 CD19+ healthy donor peripheral blood samples.
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| Contributor(s) |
Stopka T, Trněný M, Vargová K, Zavadil J, Kulvait V |
| Citation(s) |
21296997 |
| Submission date |
Jan 19, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Vojtěch Kulvait |
| E-mail(s) |
vojtech.kulvait@lf1.cuni.cz
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| Phone |
+420224965968
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| Organization name |
Charles University in Prague - 1st Faculty of Medicine
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| Department |
Institute of Pathological Physiology
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| Lab |
Centrum for experimental haematology
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| Street address |
U Nemocnice 5
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| City |
Prague 2 |
| ZIP/Postal code |
128 53 |
| Country |
Czech Republic |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA136137 |
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