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| Status |
Public on Jul 21, 2011 |
| Title |
Determination of the relative abundance of mRNAs in a polyribosome-associated pool |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Polyribosomal fractions derived from P25 mice were the starting material for IPs of the FMRP protein and associated mRNA. Therefore we wanted to determine the relative abundance of all mRNAs in the starting pool for the IP as a denominator with which to compare the IPed material.
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| Overall design |
[mRNA profiling]: We prepared polyribosomes on sucrose gradients in duplicate from 2 FVB WT mice, age P25, and purified RNA from polyribosomal fractions of each of the two gradients.
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| Contributor(s) |
Darnell RB, Darnell JC, Zhang C, Fak JJ |
| Citation(s) |
21784246 |
| Submission date |
Jan 20, 2011 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Chaolin Zhang |
| E-mail(s) |
czhang@rockefeler.edu
|
| Organization name |
Rockefeller University
|
| Department |
Molecular Neuro-Oncology
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE26809 |
FMRP Associates with Polyribosomes |
|
| Relations |
| BioProject |
PRJNA142131 |
