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| Status |
Public on Dec 08, 2011 |
| Title |
siRNA knockdown of ribosomal protein gene RPL19 abrogates the aggressive phenotype of human prostate cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Ribosomal protein RPL19 is an integral component of eukaryotic ribosomes and universally involved in protein synthesis. Although consistently stable in normal cells, RPL19 transcription is relatively elevated in prostate cancer. Using siRNA, we inhibited RPL19 transcription and demonstrated the gene to be functionally involved in promoting the malignant phenotype. Reducing RPL19 modulates a subset of genes rather than globally down-regulating protein synthesis, as evidenced by Western blotting and maintenance of cell proliferation. Mechanistically, either all ribosomes are not structurally and functionally identical or absence of RPL19 is compensated by other ribosomal protein genes. Following RPL19 inhibition, gene expression analysis confirms induction of the non-malignant phenotype principally to involve perturbation of transcription factor networks and cellular adhesion genes.
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| Overall design |
10 microarray experiments were analysed using five different prostate cell types: PC3M, PNT2, PC3M with stable knockdown transfection, PC3M with a scrambled virus and various PC3M knockdowns pooled
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| Contributor(s) |
Bee A, Brewer D, Lane B, Beesley C, Dodson A, Forootan S, Dickinson T, Gerald P, Yao S, Cooper CS, Gosden CM, Ke Y, Djamgoz MB, Foster CS |
| Citation(s) |
21799931 |
| Submission date |
Jan 20, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Ian Giddings |
| E-mail(s) |
ian.giddings@icr.ac.uk, daniel.brewer@icr.ac.uk
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| Phone |
+44 20 8722 4293
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| Fax |
+44 20 8722 4141
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| URL |
http://www.crukdmf.icr.ac.uk
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| Organization name |
Institute of Cancer Research
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| Department |
Section of Molecular Carcinogenesis
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| Lab |
CANCER RESEARCH UK DNA Microarray Facility
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| Street address |
15 Cotswold Road
|
| City |
Sutton |
| State/province |
Surrey |
| ZIP/Postal code |
SM2 5NG |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA136283 |
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