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Series GSE26790 Query DataSets for GSE26790
Status Public on Apr 18, 2012
Title The HDAC inhibitor panobinostat (LBH589) inhibits Acute Lymphoblastic leukemia (ALL) in vitro and in vivo in a new characterized human ALL mice model (TOM-1 and MOLT-4)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Histone deacetylases (HDACs) have been identified as therapeutic targets due to regulatory function in DNA structure and organization. We have analyzed the role of the LBH589, a novel pan inhibitor of class I and II HDACs, in Acute Lymphoblastic Leukemia. In vitro, LBH589 was shown to induce a dose dependent antiproliferative and apoptotic effect which was associated with an increase in the acetylation of H3 and H4 histone acetylation which was uniformly in every genetic subgroup of ALL. In vivo administration of LBH589 in BALB/c-RAG2-/-γc-/- mice in which T and B-cell leukemic cell lines were injected induced a significant reduction in tumor growth (TOM-1, p<0.01 and MOLT-4 p<0.05). Leukemic cells from patients were employed to establish a xenograft model of human leukemia in BALB/c-RAG2-/-γc-/- mice and further transplanted in consecutive generations of mice. Treatment of these xenografts with LBH589 induced an increase in the acetylation of H3 and H4 and prolonged the survival of mice in comparison with the animals treated with Vincristine and Dexametasone (p<0.05) and this effect was significantly higher when LBH589 was combined with Vincristine and Dexametasone (p<0.001). Our results that the use of LBH589 in combination with standard chemotherapy represents an attractive option for treatment of patients with ALL.
 
Overall design Three different biological replicates of ALL derive cell lines TOM-1 and MOLT-4 after and before treatment with LBH589
 
Contributor(s) Vilas-Zornoza A, Agirre X, Abizanda G, Moreno C, Segura V, Rodriguez AD, San José-Eneriz E, Blanco-Prieto5 M, García de Jalón JA, Rifón J, Cigudosa JC, Martinez-Climent JA, Román-Gómez J, Calasanz MJ, Prósper F
Citation(s) 22307227
Submission date Jan 21, 2011
Last update date Jul 26, 2018
Contact name Xabier Agirre
E-mail(s) xaguirre@unav.es
Phone +34 948194700
Organization name CIMA
Department Oncology
Street address Pio XII, 55
City Pamplona
State/province Navarra
ZIP/Postal code 31008
Country Spain
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (12)
GSM659132 ALL derived MOLT-4 cell line control T1
GSM659133 ALL derived MOLT-4 cell line control T2
GSM659134 ALL derived MOLT-4 cell line control T3
This SubSeries is part of SuperSeries:
GSE26807 The HDAC inhibitor panobinostat (LBH589) inhibits Acute Lymphoblastic leukemia (ALL) in vitro and in vivo in a new characterized human ALL mice model
Relations
BioProject PRJNA142115

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26790_RAW.tar 52.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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