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| Status |
Public on Mar 01, 2012 |
| Title |
Expression data from GFAP-negative LC cells exposed to 24 hours of hypoxia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Vascular hypoperfusion is a pathological phenomenon in the glaucomatous optic nerve head. We report transcriptional responses in GFAP-negative LC cells exposed to in-vitro hypoxic stress (1%O2).
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| Overall design |
The study design was as follows:
For GSM659595: "human donor 1" LC cells were passaged into three p100 plates of cells and then exposed to normoxia for 24 hours. The RNA from each of these three plates was then extracted, pooled and hybridised to the microarray "normoxia_1".
For GSM659592: "human donor 1" LC cells were passaged into three p100 plates of cells and then exposed to hypoxia for 24 hours. The RNA from each of these three plates was then extracted, pooled and hybridised to the microarray "hypoxia_1".
"hypoxia_1" and "normoxia_1" microarray data were then compared to calculate differential gene expression in response to hypoxia.
This experiment (same design) was then conducted a second and third time using biologically independent "human donor 2" and "human donor 3" cell lines respectively.
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| Contributor(s) |
Kirwan RP, Felica L, Clark AF, O'Brien CJ, Leonard MO |
| Citation(s) |
22427556 |
| Submission date |
Jan 24, 2011 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Ruaidhri Patrick Kirwan |
| E-mail(s) |
ruaidhri.kirwan@ucd.ie
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| Organization name |
University College Dublin
|
| Department |
Conway Institute of Biomolecular and Biomedical Research & School of Medicine and Medical Science
|
| Lab |
Dr. Martin Leonard
|
| Street address |
Belfield
|
| City |
Dublin |
| State/province |
Leinster |
| ZIP/Postal code |
D4 |
| Country |
Ireland |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (6)
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| GSM659592 |
LC cells exposed to hypoxic stress |
| GSM659593 |
LC cells exposed to hypoxic stress (biological replicate 1) |
| GSM659594 |
LC cells exposed to hypoxic stress (biological replicate 2) |
| GSM659595 |
Normoxic control LC cells |
| GSM659596 |
Normoxic control LC cells (biological replicate 1) |
| GSM659597 |
Normoxic control LC cells (biological replicate 2) |
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| Relations |
| BioProject |
PRJNA136019 |
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