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Status |
Public on Jan 25, 2011 |
Title |
The codon 72 polymorphism of p53 regulates interactions with NF-κB and transactivation of genes involoved in immunity and inflamation |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
A common polymorphism at codon 72 in the p53 tumor suppressor gene encodes either proline(P72) or arginine(R72). These two variants might possess altered biological function which influences p53’s transcriptional, senescence and apoptotic functions. We found that P72 has increased apoptosis when compare to R72. To further identify unknown p53 target genes which might play a role in the increased apoptosis in P72 thymocytes, we have employed whole genome microarray expression profiling as a discovery platform. Mouse thymocytes were isolated from the thymuses of mice that were untreated (0 hour) or following 2 or 4 hours of exposure to 5 Gray of gamma radiation.
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Overall design |
Mouse thymocytes were isolated from the thymuses of mice that were untreated (0 hour) or following 2 or 4 hours of exposure to 5 Gray of gamma radiation. Four independent experiments were performed at each time point (P72 and R72) and at each timepoint 2 mice of each genotype were used, and their thymocytes were pooled together.
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Contributor(s) |
Frank AK, Leu JL, Zhou Y, Devarajan K, Nedelko T, Klein-Szanto A, Hollstein M, Murphy ME |
Citation(s) |
21245379 |
Submission date |
Jan 25, 2011 |
Last update date |
Jan 12, 2017 |
Contact name |
Yan Zhou |
E-mail(s) |
yan.zhou@fccc.edu
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Organization name |
Fox Chase Cancer Center
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Department |
Bioinformatics
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Street address |
333 Cottman Ave
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City |
3601 Spruce Street |
State/province |
PA |
ZIP/Postal code |
19111 |
Country |
USA |
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Platforms (1) |
GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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Samples (32)
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Relations |
BioProject |
PRJNA135973 |