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| Status |
Public on Nov 12, 2011 |
| Title |
Time kinetics of gene expression in NK92 cells after Plasmodium falciparum-iRBC encounter |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To study the effect of Plasmodium falciparum-infected erythrocytes on gene expression in NK92 cells, microarray analysis after 6, 12 and 24 hours of co-culture with either uRBC or iRBC was performed. The aim was to identify pathways in NK92 cells that are switched on after iRBC encounter in a time-dependent manner that will help to understand the mechanisms in innate immune defenses against Plasmodium falciparum infection.
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| Overall design |
Variation in gene expression of NK92 cells was determined after 6, 12, and 24 hours of co-culture with either infected or uninfected RBC compared to timepoint 0 (start of co-culture, untreated control). All experiments were done in triplicate, that means that samples were collected in 3 different experiments (A, B, and C) each time after 0, 6, 12 and 24 hours of co-culture.
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| Contributor(s) |
Boettger E |
| Citation(s) |
22018162 |
| Submission date |
Jan 26, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Evelyn Boettger |
| E-mail(s) |
evelyn-boettger@hotmail.de
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| Organization name |
Institute of Tropical Medicine
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| Street address |
Wilhelmstrasse 27
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| City |
Tuebingen |
| ZIP/Postal code |
72074 |
| Country |
Germany |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (21)
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| GSM661509 |
NK92 + uRBC (12h, A) |
| GSM661511 |
NK92 + iRBC (12h, A) |
| GSM661512 |
NK92 + uRBC (24h, A) |
| GSM661513 |
NK92 + iRBC (24h, A) |
| GSM661514 |
NK92 before co-culture (0h, B) |
| GSM661518 |
NK92 + uRBC (6h, B) |
| GSM661519 |
NK92 + iRBC (6h, B) |
| GSM661520 |
NK92 + uRBC (12h, B) |
| GSM661521 |
NK92 + iRBC (12h, B) |
| GSM661522 |
NK92 + uRBC (24h, B) |
| GSM661525 |
NK92 + iRBC (24h, B) |
| GSM661526 |
NK92 before co-culture (0h, C) |
| GSM661527 |
NK92 + uRBC (6h, C) |
| GSM661528 |
NK92 + iRBC (6h, C) |
| GSM661529 |
NK92 + uRBC (12h, C) |
| GSM661531 |
NK92 + iRBC (12h, C) |
| GSM661532 |
NK92 + uRBC (24h, C) |
| GSM661545 |
NK92 + iRBC (24h, C) |
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| Relations |
| BioProject |
PRJNA136117 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26876_RAW.tar |
85.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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