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Series GSE26918 Query DataSets for GSE26918
Status Public on Oct 26, 2011
Title Total gene expression analysis of LMP2A/λ-MYC and λ-MYC tumors
Organism Mus musculus
Experiment type Expression profiling by array
Summary We have developed a transgenic mouse model for Epstein-Barr virus-associated Burkitt's lymphoma. Transgenic mice that express LMP2A and MYC in B cells develop spontaneous lymph node tumors at different rates. Tumor onset occurs at approximately 40-60 days in LMP2A/λ-MYC mice and at 100-200 days in λ-MYC mice. This study compared total gene expression in the tumor cells from each genotype, as well as in B cells isolated from 3 week old mice prior to tumor onset. Comparison of total gene expression in tumor cells from LMP2A/λ-MYC and λ-MYC transgenic mice identified a short list of differentially expressed genes. Comparison of gene expression in B cells from the spleens of 3 week old mice, in contrast, identified a 10-fold increase in the number of differentially expressed genes.
 
Overall design B cells from the spleens of 3 week old LMP2A/λ-MYC (n=5), λ-MYC (n=5), LMP2A (n=3), and WT (n=3) transgenic mice were purified using magnetic-activated cell sorting (MACS) beads purchased from Miltenyi for CD19 positive selection. Total RNA was isolated from these purified B cells, as well as from cervical lymph node tumor cells that developed in LMP2A/λ-MYC (n=7) and λ-MYC (n=5) transgenic mice using RNeasy RNA extraction kit from QIAGEN. Cervical lymph node tumor cells were >90% positive for the B cell marker B220, and were not further purified. Total gene expression was assessed in each sample, along with heart and brain reference samples using MouseRef-8 v2.0 Expression Bead Chips purchased from Illumina. GeneSpring analysis software was used to analyze the expression data and identify significantly differentially expressed genes (fold change >1.5-fold and false discovery rate< 0.05).
 
Contributor(s) Bieging KT, Longnecker R
Citation(s) 22039254
Submission date Jan 27, 2011
Last update date Jun 14, 2018
Contact name Kathryn Bieging
Organization name Northwestern University
Department Microbiology-Immunology
Lab Longnecker
Street address 303 East Chicago Ave
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (28)
GSM662840 LMP2A/λ-MYC cervcial lymph node tumor mouse 5850
GSM662841 LMP2A/λ-MYC cervcial lymph node tumor mouse 5662
GSM662842 LMP2A/λ-MYC cervcial lymph node tumor mouse 5646
Relations
BioProject PRJNA136181

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26918_RAW.tar 3.1 Mb (http)(custom) TAR
GSE26918_non-normalized.txt.gz 2.9 Mb (ftp)(http) TXT
GSE26918_normalized.txt.gz 3.5 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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