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Series GSE27238 Query DataSets for GSE27238
Status Public on Apr 17, 2011
Title FACS-array profiling in retinal endothelial cells from living mouse retinas
Organism Mus musculus
Experiment type Expression profiling by array
Summary Deregulated retinal angiogenesis directly cause vision loss in many ocular diseases, such as diabetic retinopathy and retinopathy of prematurity. To identify endothelial-specific genes expressed in angiogenic retinal vessels, we purified genetically labeled endothelial cells from Tie2-GFP transgenic mice and performed gene expression profiling using DNA microarray. To find out genes associated with angiogenesis, comparisons of microarray data were carried out between GFP-negative non-endothelial retinal cells and GFP-positive retinal endothelial cells in angiogenic P8 retina.
 
Overall design Eighteen arrays are included. Utilizing fluorescence-activated cell sorting (FACS), we isolated endothelial cells as GFP-positive cells from P8 retina in homozygous Tie2-GFP transgenic mice. GFP-negative cells were served as non-endothelial control. RNA extracts from sorted cells were amplified and then hybridized to Affymetrix MGU74v2 series arrays in triplicate. 
 
Contributor(s) Jakt LM
Citation(s) 21505259, 23029280, 28194443
Submission date Feb 11, 2011
Last update date Feb 18, 2018
Contact name Sentaro Kusuhara
E-mail(s) kusu@med.kobe-u.ac.jp
Phone +81-78-382-6048
Organization name Kobe University Graduate School of Medicine
Department Department of Surgery
Lab Division of Ophthalmology
Street address 7-5-2 Kusunoki-cho, Chuo-ku
City Kobe
State/province Hyogo
ZIP/Postal code 650-0017
Country Japan
 
Platforms (3)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL82 [MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array
GPL83 [MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array
Samples (18)
GSM673567 GFP-pos_P8_A_rep1
GSM673568 GFP-pos_P8_A_rep2
GSM673569 GFP-pos_P8_A_rep3
Relations
BioProject PRJNA137491

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27238_RAW.tar 45.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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