|
| Status |
Public on Nov 21, 2011 |
| Title |
Pitx1 Haploinsufficiency Causes Clubfoot in Humans and Mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify genes regulated by Pitx1 during early hindlimb development, we compared gene expression differences between hindlimb buds from E12.5 Pitx1+/+ and Pitx1-/- mice on the Illumina MouseRef-8 expression Bead Chip. Genes involved in muscle development GeneGO processes were highly enriched in our dataset, specifically in down-regulated genes. This data is consistent with the reduced muscle volumes that were observed in the clubfoot limbs of our adult mice.
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| |
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| Overall design |
Hindlimb buds were collected from Pitx1+/- mated mice at embryonic day E12.5 and genotypes were determined by PCR of DNA isolated from tails.
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| Contributor(s) |
Alvarado DM, McCall K, Aferol H, Silva M, Garbow J, Siegel M, Dobbs MB, Gurnett CA |
| Citation(s) |
21775501 |
| Submission date |
Feb 16, 2011 |
| Last update date |
Jun 14, 2018 |
| Contact name |
David Michael Alvarado |
| E-mail(s) |
alvaradod@wudosis.wustl.edu
|
| Phone |
314 454-4089
|
| Fax |
314 454-4225
|
| Organization name |
Washington University in St. Louis
|
| Department |
Orthopaedic Surgery
|
| Street address |
660 South Euclid
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA137031 |
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