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Series GSE27363 Query DataSets for GSE27363
Status Public on Nov 21, 2011
Title Pitx1 Haploinsufficiency Causes Clubfoot in Humans and Mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary To identify genes regulated by Pitx1 during early hindlimb development, we compared gene expression differences between hindlimb buds from E12.5 Pitx1+/+ and Pitx1-/- mice on the Illumina MouseRef-8 expression Bead Chip. Genes involved in muscle development GeneGO processes were highly enriched in our dataset, specifically in down-regulated genes. This data is consistent with the reduced muscle volumes that were observed in the clubfoot limbs of our adult mice.
 
Overall design Hindlimb buds were collected from Pitx1+/- mated mice at embryonic day E12.5 and genotypes were determined by PCR of DNA isolated from tails.
 
Contributor(s) Alvarado DM, McCall K, Aferol H, Silva M, Garbow J, Siegel M, Dobbs MB, Gurnett CA
Citation(s) 21775501
Submission date Feb 16, 2011
Last update date Jun 14, 2018
Contact name David Michael Alvarado
E-mail(s) alvaradod@wudosis.wustl.edu
Phone 314 454-4089
Fax 314 454-4225
Organization name Washington University in St. Louis
Department Orthopaedic Surgery
Street address 660 South Euclid
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM676359 WT-1
GSM676360 WT-2
GSM676361 WT-3
Relations
BioProject PRJNA137031

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27363_RAW.tar 3.1 Mb (http)(custom) TAR
GSE27363_non-normalized.txt.gz 1.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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