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Series GSE27569 Query DataSets for GSE27569
Status Public on Mar 01, 2011
Title Expression data from zebrafish depleted of Esco2
Organism Danio rerio
Experiment type Expression profiling by array
Summary Our study in zebrafish is the first to use an animal model to understand the biology of the developmental disorder Roberts Syndrome (RBS). RBS is caused by mutations in the ESCO2 gene.
We have used morpholinos (MO) to knock down esco2 in zebrafish to better understand the pathology of this rare human syndrome. Our zebrafish model nicely phenocopies the developmental defects observed in RBS.
Overall design RNA from esco2 morphants and control (injected with buffer only) zebrafish embryos collected at 24 hours post-fertilization (h.p.f.) and 48 h.p.f. was hybridized to Affymetrix microarrays (Gene Chip zebrafish genome arrays cat. no. 900488). Four pools of 50 embryos for each genotype and time point were used as the RNA source, and RNA from each pool was hybridized independently such that the experiment had four biological replicates. Note that the chip for the 2nd replicate of the control embryos at 48 h.p.f. was faulty and the data is therefore not included.
Contributor(s) Mönnich M, McNoe L, Print CG, Horsfield JA
Citation(s) 21637801
Submission date Feb 28, 2011
Last update date Jan 25, 2018
Contact name Julia Horsfield
Organization name University of Otago
Department Pathology Department
Lab Chromosome Structure and Development Group
Street address Cnr Hanover and Great King Streets
City Dunedin
ZIP/Postal code 9016
Country New Zealand
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (15)
GSM683599 control embryos at 24hpf, biological rep 1
GSM683600 control embryos at 24hpf, biological rep 2
GSM683601 control embryos at 24hpf, biological rep 3
BioProject PRJNA138409

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Supplementary file Size Download File type/resource
GSE27569_RAW.tar 30.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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