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Series GSE27688 Query DataSets for GSE27688
Status Public on Sep 05, 2012
Title Gene expression and epigenetic profiling of CD34+ hematopoietic progenitor cells in multiple sclerosis patients (expression)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Intense immunosuppression followed by autologous hematopoietic stem cell transplantation (aHSCT) is a potential treatment for patients suffering from aggressive multiple sclerosis (MS). However it remains unresolved whether autologous CD34+ hematopoietic progenitor cells of MS patients show gene expression differences prior to aHSCT that indicate a preset proinflammatory state, which would then also predispose to or predetermine recurrence of the autoimmune disease. To approach this key point we compared the gene expression signature of CD34+ and CD34- cells collected from MS patients and healthy donors (HD). Whole genome gene expression of CD34+ and CD34- cells was analysed with the Human 4x44K Design Array (Agilent-Technologies). As main observation we found only minor differences in the gene expression signature of MS patients compared to HD. Only a single gene, troponin-type-1 (TNNT1), reached statistical significance after correction for multiple comparisons (logFC=3.1, p<0.01). There was a decreased expression of several protease genes, myeloperoxidase (MPO), neutrophil-elastase (ELA2), cathepsin-G (CTSG) and serine-protease 21 (PRSS21) in HPCs of MS patients, albeit not reaching statistical significance. In summary we did not detect substantial alterations in the gene expression profile of CD34+ HPCs in MS. Our data support the use of autologous hematopoietic stem cell transplantation for treatment of an autoimmune disease.
 
Overall design Samples of CD34+ cells were obtained from 4 female MS patients and 4 age matched healthy donors (3 female) mobilized by G-CSF (2x5μg/kg/day) and 4 MS patients (2 female) mobilized by G-CSF (5μg/kg/day) plus Cyclophosphamide (Cy, 4g/m2). White blood cells, containing the CD34+ cell fraction, were collected by leucocytapheresis from peripheral blood, frozen and stored in liquid nitrogen. All samples were thawed and processed at one center and CD34+ HPCs purified by magnetic bead separation using the autoMACS system (Miltenyi). Purity and viability of CD34+ cells was analyzed by Fluorescence Activated Cell Sorter (FACS). Total cellular RNA were extracted with TRIzol reagent and analyzed with the Human 4x44K Design Array (Agilent-Technologies).
 
Contributor(s) Lutterotti A, Jelcic I, Schulze C, Schippling S, Breiden P, Mazzanti B, Reinhard S, DiGioia M, Repice A, Massacesi L, Sputtek A, Salinas-Riester G, Kroeger N, Sospedra M, Saccardi R, Zander A, Martin R
Citation(s) 22252466
Submission date Mar 04, 2011
Last update date Jan 23, 2019
Contact name Christian Schulze
E-mail(s) christian.schulze@zmnh.uni-hamburg.de
Organization name ZMNH
Street address Falkenried 94
City Hamburg
ZIP/Postal code 20251
Country Germany
 
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (30)
GSM685767 CD34neg_ControlDonor_A
GSM685768 CD34neg_ControlDonor_B
GSM685769 CD34neg_ControlDonor_C
This SubSeries is part of SuperSeries:
GSE27694 Gene expression and epigenetic profiling of CD34+ hematopoietic progenitor cells in multiple sclerosis patients
Relations
BioProject PRJNA141875

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27688_RAW.tar 270.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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