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Status |
Public on Sep 05, 2012 |
Title |
Gene expression and epigenetic profiling of CD34+ hematopoietic progenitor cells in multiple sclerosis patients (expression) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Intense immunosuppression followed by autologous hematopoietic stem cell transplantation (aHSCT) is a potential treatment for patients suffering from aggressive multiple sclerosis (MS). However it remains unresolved whether autologous CD34+ hematopoietic progenitor cells of MS patients show gene expression differences prior to aHSCT that indicate a preset proinflammatory state, which would then also predispose to or predetermine recurrence of the autoimmune disease. To approach this key point we compared the gene expression signature of CD34+ and CD34- cells collected from MS patients and healthy donors (HD). Whole genome gene expression of CD34+ and CD34- cells was analysed with the Human 4x44K Design Array (Agilent-Technologies). As main observation we found only minor differences in the gene expression signature of MS patients compared to HD. Only a single gene, troponin-type-1 (TNNT1), reached statistical significance after correction for multiple comparisons (logFC=3.1, p<0.01). There was a decreased expression of several protease genes, myeloperoxidase (MPO), neutrophil-elastase (ELA2), cathepsin-G (CTSG) and serine-protease 21 (PRSS21) in HPCs of MS patients, albeit not reaching statistical significance. In summary we did not detect substantial alterations in the gene expression profile of CD34+ HPCs in MS. Our data support the use of autologous hematopoietic stem cell transplantation for treatment of an autoimmune disease.
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Overall design |
Samples of CD34+ cells were obtained from 4 female MS patients and 4 age matched healthy donors (3 female) mobilized by G-CSF (2x5μg/kg/day) and 4 MS patients (2 female) mobilized by G-CSF (5μg/kg/day) plus Cyclophosphamide (Cy, 4g/m2). White blood cells, containing the CD34+ cell fraction, were collected by leucocytapheresis from peripheral blood, frozen and stored in liquid nitrogen. All samples were thawed and processed at one center and CD34+ HPCs purified by magnetic bead separation using the autoMACS system (Miltenyi). Purity and viability of CD34+ cells was analyzed by Fluorescence Activated Cell Sorter (FACS). Total cellular RNA were extracted with TRIzol reagent and analyzed with the Human 4x44K Design Array (Agilent-Technologies).
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Contributor(s) |
Lutterotti A, Jelcic I, Schulze C, Schippling S, Breiden P, Mazzanti B, Reinhard S, DiGioia M, Repice A, Massacesi L, Sputtek A, Salinas-Riester G, Kroeger N, Sospedra M, Saccardi R, Zander A, Martin R |
Citation(s) |
22252466 |
Submission date |
Mar 04, 2011 |
Last update date |
Jan 23, 2019 |
Contact name |
Christian Schulze |
E-mail(s) |
christian.schulze@zmnh.uni-hamburg.de
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Organization name |
ZMNH
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Street address |
Falkenried 94
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City |
Hamburg |
ZIP/Postal code |
20251 |
Country |
Germany |
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Platforms (1) |
GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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Samples (30)
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This SubSeries is part of SuperSeries: |
GSE27694 |
Gene expression and epigenetic profiling of CD34+ hematopoietic progenitor cells in multiple sclerosis patients |
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Relations |
BioProject |
PRJNA141875 |
Supplementary file |
Size |
Download |
File type/resource |
GSE27688_RAW.tar |
270.9 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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