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Series GSE27851 Query DataSets for GSE27851
Status Public on Jan 01, 2014
Title The persistent milk yield response to frequent milking during early lactation is associated with persistent changes in mammary gene expression
Organism Bos taurus
Experiment type Expression profiling by array
Summary BACKGROUND:
The lactating mammary gland responds to changes in milking frequency by modulating milk production. This response is locally regulated and, in dairy cows, the udder is particularly sensitive during early lactation. Relative to cows milked twice-daily throughout lactation, those milked four-times-daily for just the first 3 weeks of lactation produce more milk throughout that lactation. We hypothesized that the milk yield response would be associated with increased mammary cell turnover and changes in gene expression during frequent milking and persisting thereafter. Cows were assigned to unilateral frequent milking (UFM; left udder halves milked twice-daily; right udder halves milked four-times daily) on days 1 to 21 of lactation, followed by twice-daily milking for the remainder of lactation. Relative to udder halves milked twice-daily, those milked four-times produced more milk during UFM; the difference in milk yield declined acutely upon cessation of UFM after day 21, but remained significantly elevated thereafter. We obtained mammary biopsies from both udder halves on days 21, 23, and 40 of lactation.

RESULTS:
Mammary cell proliferation and apoptosis were not affected by milking frequency. We identified 75 genes that were differentially expressed between paired udder halves on day 21 but exhibited a reversal of differential expression on day 23. Among those genes, we identified four clusters characterized by similar temporal patterns of differential expression. Two clusters (11 genes) were positively correlated with changes in milk yield and were differentially expressed on day 21 of lactation only, indicating involvement in the initial milk yield response. Two other clusters (64 genes) were negatively correlated with changes in milk yield. Twenty-nine of the 75 genes were also differentially expressed on day 40 of lactation.

CONCLUSIONS:
Changes in milking frequency during early lactation did not alter mammary cell population dynamics, but were associated with coordinated changes in mammary expression of at least 75 genes. Twenty-nine of those genes were differentially expressed 19 days after cessation of treatment, implicating them in the persistent milk yield response. We conclude that we have identified a novel transcriptional signature that may mediate the adaptive response to changes in milking frequency.
 
Overall design 36 samples from 6 cows; 3 timepoints
 
Contributor(s) Wall EH, Bond JP, McFadden TB
Citation(s) 23638659
Submission date Mar 09, 2011
Last update date Jun 11, 2014
Contact name Emma Hull Wall
E-mail(s) emma.wall@uvm.edu
Phone 802-318-1731
Organization name University of Vermont
Department Medicine
Street address 89 Beaumont Ave
City Burlington
State/province VT
ZIP/Postal code 05405
Country USA
 
Platforms (1)
GPL2112 [Bovine] Affymetrix Bovine Genome Array
Samples (36)
GSM687370 mammary gland_control_21 d_rep1
GSM687371 mammary gland_four times daily milking 21 d_rep 1
GSM687372 mammary gland_control_23 d_rep1
Relations
BioProject PRJNA138051

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Supplementary file Size Download File type/resource
GSE27851_RAW.tar 70.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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