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Series GSE27860 Query DataSets for GSE27860
Status Public on Jan 10, 2012
Title Genome wide analysis reveals association of a FTO gene variant with epigenetic changes of several genes in human blood
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary FTO gene variants show the strongest association to obesity so far, but the role of the gene in obesity remains unclear. The FTO protein is a demethylation enzyme, which suggests that the link to obesity could be mediated through epigenetic changes. Herein, we determine the genome wide DNA methylation profile in blood from 47 female preadolescents and detect changes in five methylated regions, corresponding to six genes, associated with the FTO risk allele (rs9939609). In addition, we identify 20 differentially methylated regions associated with obesity. Our findings indicate that the effect of the FTO obesity risk allele may be mediated through epigenetic changes. Further, the identified regions might prove valuable biomarkers for the diagnosis and prognosis of obesity and comorbidites, such as cardiovascular disease.
 
Overall design Bisulphite converted DNA from 47 girls were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2. Half of the subjects are carriers of the FTO obesity risk allele (AA) and half of the normal allele (TT). In addition, both obese and normal-weigh indiviudals were included.
 
Contributor(s) Almén MS, Jacobsson JA, Moschonis G, Benedict C, Chrousos GP, Fredriksson R, Schiöth HB
Citation(s) 22234326
Submission date Mar 09, 2011
Last update date Jan 02, 2015
Contact name Markus Sällman Almén
Organization name Uppsala Universitet
Department Dept. of Neuroscience
Lab Functional Pharmacology
Street address Box 593
City Uppsala
ZIP/Postal code 75124
Country Sweden
 
Platforms (1)
GPL8490 Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2)
Samples (47)
GSM687742 normal-weight female child with FTO allele TT N_girl_12
GSM687743 normal-weight female child with FTO allele AA N_girl_13
GSM687744 normal-weight female child with FTO allele AA N_girl_14
Relations
BioProject PRJNA137883

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27860_RAW.tar 5.8 Mb (http)(custom) TAR
GSE27860_signal_intensities.txt.gz 6.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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