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| Status |
Public on Apr 21, 2011 |
| Title |
Expression data from C. elegans (wild type vs. tag-24) |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
To provide insights into the mechanism underlying the enhanced immunity of tag-24/octr-1 animals, we used genome microarrays to find clusters of genes commonly misregulated in tag-24 relative to wild-type animals grown on live P. aeruginosa.
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| Overall design |
Gravid adult wild-type and tag-24/octr-1 animals were lysed using a solution of sodium hydroxide and bleach (ratio of 5:2), washed and the eggs were synchronized for 22 hours in S basal liquid medium at room temperature. Synchronized L1 larval animals were placed onto NGM plates seeded with E. coli OP50 and grown at 20°C until the animals had reached the L4 larval stage. Animals were collected and washed with M9 buffer before transferring to NGM plates containing P. aeruginosa PA14 for 4 hours at 25°C. After 4 hours, animals were collected and washed with M9 buffer, and RNA was extracted using TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment (Ambion Austin, TX).
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| Contributor(s) |
Aballay A |
| Citation(s) |
21474712 |
| Submission date |
Mar 09, 2011 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Alejandro Aballay |
| Organization name |
Duke University
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Aballay
|
| Street address |
268 Jones
|
| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA138015 |
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