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Series GSE27903 Query DataSets for GSE27903
Status Public on Jul 13, 2011
Title The effects of the 5' pocket motif of Dicer on miRNA biogenesis
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary A hallmark of RNA silencing is a class of ~22 nt RNAs which are processed from dsRNA precursor by Dicer. Accurate processing by Dicer is critical for the functionality of microRNAs (miRNAs). According to the current model, Dicer measures the length by anchoring the 3' overhang of the dsRNA terminus. Here we find that human Dicer binds to the 5' end of RNA and utilizes the 5' end as an additional reference point for cleavage site selection (5' counting rule). We further identify a novel motif (5'-pocket) in Dicer, which recognizes the 5' end of RNA. By analyzing miRNA population from 5'-pocket mutant Dicer expressing Dicer-null mES, we provide that the 5' pocket is significant for Dicer processing in vivo.
Overall design Examination of small RNA profiles from Dicer-null mouse embryonic stem cells transfected with either wild-type or 5' pocket mutant Dice expression plasmids.
Web link
Contributor(s) Park J, Heo I, Chang H, Kim VN
Citation(s) 21753850
Submission date Mar 11, 2011
Last update date Jul 20, 2018
Contact name Hyeshik Chang
Phone +82-2-887-1343
Organization name Institute for Basic Science
Department Center for RNA Research
Lab Narry Kim Lab
Street address Building 504 Room 531, School of Biological Sciences, Seoul National University, 599 Gwanangno, Gwanak-gu
City Seoul
State/province South Korea
ZIP/Postal code 08826
Country South Korea
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (5)
GSM689053 wt_rep1
GSM689054 wt_rep2
GSM689055 5pmut_rep1
BioProject PRJNA138215

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Processed data included within Sample table

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