|
| Status |
Public on Mar 23, 2011 |
| Title |
Examination of hepatic SREBP-2 binding using ChIP-Seq |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Sterol regulatory element binding proteins (SREBPs) are key transcriptional regulators of lipid metabolism. To define functional differences between the three mammalian SREBPs we are using genome-wide ChIP-seq with isoform-specific antibodies and chromatin from select tissues of mice challenged with different dietary conditions that enrich for specific SREBPs. We show hepatic SREBP-2 binds preferentially to two different gene-proximal motifs. Gene ontology analyses suggests SREBP-2 targets lipid metabolic processes as expected but apoptosis and autophagy gene categories were also enriched. We show SREBP-2 directly activates autophagy genes during cell sterol depletion, conditions known to induce both autophagy and nuclear SREBP-2 levels. Additionally, SREBP-2 knockdown during nutrient depletion decreased autophagosome formation and lipid droplet association of the autophagosome targeting protein LC3. Thus, the lipid droplet could be viewed as a third source of cellular cholesterol, which along with sterol synthesis and uptake, is also regulated by SREBP-2.
|
| |
|
| Overall design |
Examination of hepatic SREBP-2 binding using ChIP-Seq. One ChIP-Seq dataset and one IgG control.
|
| |
|
| Contributor(s) |
Seo Y, Jeon T, Chong H, Biesinger J, Xie X, Osborne TF |
| Citation(s) |
21459322 |
| Submission date |
Mar 22, 2011 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Jacob Biesinger |
| E-mail(s) |
jake.biesinger@gmail.com
|
| Phone |
949 231 7587
|
| Organization name |
University of California, Irvine
|
| Department |
Computer Science
|
| Lab |
Xiaohui Xie
|
| Street address |
4058 Donald Bren Hall, University of California Irvine
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE28084 |
Genome-wide Localization of SREBP-2 in Hepatic Chromatin Predicts a Novel Role in Autophagy |
|
| Relations |
| BioProject |
PRJNA141747 |
