|
| Status |
Public on May 27, 2011 |
| Title |
Effect of ECAT11 disruption in mouse ESCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The principal factors that lead to proliferation and pluripotency in embryonic stem cells (ESCs) have been vigorously investigated. However, the global network of factors and their full signaling cascade is still unclear. In this study, we found that ECAT11 (L1td1) is one of the ESC-associated transcripts harboring a truncated fragment of ORF-1, a component of the L1 retrotransposable element. We generated an ECAT11 knock-in mouse by replacing its coding region with green fluorescent protein. DNA microarray analyses revealed that ECAT11EGFP/EGFP ESCs showed similar global gene expression profiles as the wild type ESCs
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| |
|
| Overall design |
Samples from wild type ESCs and ECAT11EGFP/EGFP ESCs
|
| |
|
| Contributor(s) |
Iwabuchi KA, Kumazaki M |
| Citation(s) |
21637830 |
| Submission date |
Mar 24, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
| Samples (5)
|
| GSM697303 |
RF8 [Embryonic stem cell line, wild type] |
| GSM697304 |
#620-2 [Embryonic stem cell line, wild type] |
| GSM697305 |
#620-6 [Embryonic stem cell line, ECAT11EGFP/EGFP] |
| GSM697306 |
#757-1 [Embryonic stem cell line, ECAT11EGFP/EGFP] |
| GSM697307 |
#757-2 [Embryonic stem cell line, ECAT11EGFP/EGFP] |
|
| Relations |
| BioProject |
PRJNA139559 |
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