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Series GSE28199 Query DataSets for GSE28199
Status Public on Mar 29, 2011
Title prdm1a mutant vs. wild type
Organism Danio rerio
Experiment type Expression profiling by array
Summary The PR domain containing 1a, with ZNF domain factor, gene prdm1a plays an integral role in the development of a number of different cell types during vertebrate embryogenesis, including neural crest cells, Rohon-Beard (RB) sensory neurons and the cranial neural crest-derived craniofacial skeletal elements. To better understand how Prdm1a regulates the development of various cell types in zebrafish, we performed a microarray analysis comparing wild type and prdm1a mutant embryos and identified a number of genes with altered expression in the absence of prdm1a. Rescue analysis determined that two of these, sox10 and islet1, lie downstream of Prdm1a in the development of neural crest cells and Rohon-Beard neurons, respectively. In addition, we identified a number of other novel downstream targets of Prdm1a that may be important for the development of diverse tissues during zebrafish embryogenesis.
 
Overall design RNA was isolated from whole zebrafish embryos at 25hpf, three replicates each for wildtype and prdm1a mutant embryos.
 
Contributor(s) Olesnicky E, Hernandez-Lagunas L, Artinger K
Citation(s) 20836130
Submission date Mar 28, 2011
Last update date Jan 25, 2018
Contact name KRISTIN ARTINGER
E-mail(s) kristin.artinger@ucdenver.edu
Phone 303-724-4562
Fax 303-724-4580
Organization name University of Colorado Denver
Department Craniofacial Biology
Street address 12801 East 17th St
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM698126 prdm1 mutant rep1
GSM698127 prdm1 mutant rep2
GSM698128 prdm1 mutant rep3
Relations
BioProject PRJNA139635

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28199_RAW.tar 12.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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