|
| Status |
Public on Apr 01, 2011 |
| Title |
Genome-wide maps of REST and its cofactors in mouse E14 cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the application of single-molecule-based sequencing technology for REST and its cofactors genome wide binding sites in E14 cells.We then combine these binding sirtes with REST regulating gene profiling, to understand REST binding and regulation in E14 cells.
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| |
|
| Overall design |
Examination of REST and 5 cofactors(RCOR1, RCOR2,RCOR3,SIN3A,SIN3B) in E14 cells, REST and SIN3A endogenous antibody were used for ChIP experiment. The stable E14 cells expressing low level exogenous RCOR1, RCOR2, RCOR3,and SIN3B with V5 tag were used for ChIP experiment with V5 antibody to obtain individual ChIP DNA.
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| |
|
| Contributor(s) |
Yu H, Johnson R, Kunarso G, Stanton L |
| Citation(s) |
21632747 |
| Submission date |
Mar 29, 2011 |
| Last update date |
Mar 08, 2019 |
| Contact name |
hongbing yu |
| E-mail(s) |
yuhb@gis.a-star.edu.sg
|
| Phone |
65 68088488
|
| Fax |
65 68088306
|
| Organization name |
genome institute of singapore
|
| Department |
stem cell and development
|
| Lab |
stem cell 3
|
| Street address |
60 biopolis street, #02-01, genome
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE28289 |
REST ChIP-chip and knockdown expression profiling |
|
| Relations |
| BioProject |
PRJNA143139 |
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