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Series GSE2828

Query DataSets for GSE2828

Status

Public on Aug 08, 2005

Title

The Jak-STAT signaling pathway is required but not sufficient for the antiviral response of drosophila

Organism

Drosophila melanogaster

Experiment type

Expression profiling by array

Summary

The response of drosophila to bacterial and fungal infections involves two signaling pathways, Toll and Imd, which both activate NF-kB family members. We have studied the global transcriptional response of flies to infection with drosophila C virus. Viral infection induced a set of genes distinct from those regulated by the Toll or Imd pathways, and triggered activation of a STAT binding activity. Genetic experiments showed that the JAK kinase Hopscotch was involved in the control of the viral load in infected flies, and was required, though not sufficient, for the induction of some virus-regulated genes. Our results indicate that in addition to Toll and Imd, a third evolutionary conserved innate immunity pathway operates in drosophila and counters viral infection.
Keywords: JAK/STAT virus drosophila immunity

Overall design

For each experimental challenge (Tris or DCV, 24 or 48 hours after infection), three biologically independent samples composed of 45 male Oregon R flies were used. Infection has been performed by injecting viral stocks prepared in Tris solution. Injection of the same volume of Tris has been used as control. Infected flies were then incubated for 24 or 48 hours.

Contributor(s)

Dostert C, Jouanguy E, Irving P, Troxler L, Galiana-Arnoux D, Hetru C, Hoffmann JA, Imler J

Citation(s)

16086017

Submission date

Jun 20, 2005

Last update date

Jul 06, 2016

Contact name

Jean-Luc Imler

E-mail(s)

JL.Imler@unistra.fr