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| Status |
Public on Mar 04, 2016 |
| Title |
miRNA expression data of shoot meristems of trifoliate orange |
| Platform organism |
synthetic construct |
| Sample organism |
Citrus trifoliata |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
In 1976, a spontaneous mutant derived from Poncirus trifoliata (L.) Raf with short juvenile phase, namely, precocious trifoliate orange, was found in Yichang, Hubei province, China. Compared with 6 to 8 years of the wild-type trifoliate orange, almost all of the seedlings germinated from precocious trifoliate orange only have 1 to 2 years’ juvenile period, and 20% seedlings even flowered in the year after germination. Therefore, precocious trifoliate orange is an ideal material for studying the role of miRNAs involved in citrus juvenile and adult developmental stages. To characterize these miRNAs expressed at the juvenile and adult development stages of citrus, Affymetrix miRNA arrays were used to generate miRNA profiles of shoot meristems of trifoliate orange, the results revealed that some miRNAs were down-regulated expressed at adult stage compared with juvenile stage. Detailed comparison of the expression patterns of miRNAs and corresponding target genes revealed the negative correlation between them, while few of them are positively correlated.
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| Overall design |
To investigate the expression patterns of these miRNAs at juvenile and adult stages of citrus, four independent precocious trifoliate orange RNA samples (March, June, December and April next year) were hybridized with miRNA microarray, respectively. The expression of miRNA was analyzed with the SAM software.
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| Contributor(s) |
Zhang J, Ai X, Sun L, Guo W, Peng S, Deng X, Hu C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 04, 2011 |
| Last update date |
May 02, 2017 |
| Contact name |
jin zhi zhang |
| E-mail(s) |
jinzhi327320094@126.com
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| Phone |
86-27-87281899
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| Organization name |
Huazhong Agricultural University
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| Department |
College of Horticulture and Forestry Science
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| Street address |
College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China
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| City |
WuHan |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
| GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA139425 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28363_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE28363_Summarization.csv.gz |
824.6 Kb |
(ftp)(http) |
CSV |
| Processed data included within Sample table |
| Processed data are available on Series record |
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