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| Status |
Public on Nov 14, 2005 |
| Title |
Additional systems to Prednisolone treated childhood ALL samples |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Glucocorticoids (GC) are in most chemotherapy protocols for lymphoid malignancies, particularly childhood acute lymphoblastic leukaemia (ALL) for their ability to induce apoptosis in malignant blast. The underlying mechanism, however, has so far only been investigated in model systems. This study comprises Affymetrix hgu133 plus 2.0 analyses of
Peripheral blood lymphoblasts purified at three time points (0h, 6-8h, 24h after treatment initiation) from 13 children under therapy for ALL . Treated samples were compared to untreated (0h). For comparison, expression profiles were generated from an adult ALL patient, peripheral blood lymphocytes from GC-exposed healthy donors, GC-sensitive and -resistant ALL cell lines and mouse thymocytes treated with GC in vivo and in vitro. This second series comprises the additional samples mentioned above.
Findings. An essentially complete list of GC-regulated candidate genes in clinical settings experimental and was generated, enabling immediate analysis of any gene with respect to its potential significance for GC-induced apoptosis in these systems. Gene regulations previously thought responsible for cell death in experimental systems were reconfirmed in few children only. In contrast, a small number of genes, most not implicated in GC-induced apoptosis previously, were co-ordinately regulated in the majority of children.
Keywords: treatment response
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| Overall design |
replicates: prednisolone treated GSM60594, GSM60595. untreated: GSM60597, GSM60599. Bone marrow samples sorted / unsorted: GSM60601, GSM60603.
Samples are assigned to various subgroups: sensitive systems, resistant systems, Prednisolone treated, ethanol (control) treated, cyclohexamide treated, untreated, 6 hours treatment and 24 hours treated.
Glucocorticoid treated samples of patients or cell lines or healthy donors were compared to their untreated or ethanol treated counterparts.
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| Contributor(s) |
Schmidt S, Rainer J, Riml S, Ploner C, Jesacher S, Achmüller C, Presul E, Skvortsov S, Crazzolara R, Fiegl M, Raivio T, Jänne OA, Geley S, Meister B, Kofler R |
| Citation(s) |
16293608 |
| Submission date |
Jun 28, 2005 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Johannes Rainer |
| E-mail(s) |
johannes.rainer@eurac.edu
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| Organization name |
Eurac Researc
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| Department |
Institute for Biomedicine
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| Lab |
Biomedical Informatics
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| Street address |
Via A. Volta 21
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| City |
Bolzano |
| ZIP/Postal code |
39100 |
| Country |
Italy |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA92479 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE2842_Adult-B-ALL-24h_vs_Adult-B-ALL-0h.xls |
290.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_Adult-B-ALL-6h_vs_Adult-B-ALL-0h.xls |
238.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_C-Line-C7R1dim-high-24h-GC_vs_C-Line-C7R1dim-high-6h-EtOH.xls |
803.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_C-Line-C7R1dim-high-6h-GC_vs_C-Line-C7R1dim-high-6h-EtOH.xls |
237.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_C-Line-CEMC1-ratGR-24h-GC_vs_C-Line-CEMC1-ratGR-6h-EtOH.xls |
387.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_C-Line-CEMC1-ratGR-6h-GC_vs_C-Line-CEMC1-ratGR-6h-EtOH.xls |
194.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_CHX-C7H2-6h-GC_vs_CHX-C7H2-6h-EtOH.xls |
68.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_E-Values-BiolSystems.xls |
42.3 Mb |
(ftp)(http) |
XLS |
| GSE2842_HD1-STS-24h_vs_HD1-STS-0h.xls |
185.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_HD1-STS-6h_vs_HD1-STS-0h.xls |
313.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_HD2-RPK-24h_vs_HD2-RPK-0h.xls |
173.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_HD2-RPK-6h_vs_HD2-RPK-0h.xls |
483.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_IV-B-ALL-40-24h-GC_vs_IV-B-ALL-40-24h-EtOH.xls |
497.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_IV-B-ALL-40-6h-GC_vs_IV-B-ALL-40-6h-EtOH.xls |
557.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_M-Values-BiolSystems.xls |
26.9 Mb |
(ftp)(http) |
XLS |
| GSE2842_R-Line-C7R1-6h-GC_vs_R-Line-C7R1-6h-EtOH.xls |
15.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_R-Line-C7R1dim-low-24h-GC_vs_R-Line-C7R1dim-low-6h-EtOH.xls |
150.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_R-Line-C7R1dim-low-6h-GC_vs_R-Line-C7R1dim-low-6h-EtOH.xls |
24.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_R-Line-CEMC1-24h-GC_vs_R-Line-CEMC1-6h-EtOH.xls |
242.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_R-Line-CEMC1-6h-GC_vs_R-Line-CEMC1-6h-EtOH.xls |
174.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_R-Line-PreBR4G4-24h-GC_vs_R-Line-PreBR4G4-24h-EtOH.xls |
80.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_R-Line-PreBR4G4-6h-GC_vs_R-Line-PreBR4G4-6h-EtOH.xls |
59.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_RAW.tar |
339.8 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE2842_S-Line-C7H2-24h-GC_vs_S-Line-C7H2-6h-EtOH.xls |
790.0 Kb |
(ftp)(http) |
XLS |
| GSE2842_S-Line-C7H2-6h-GC_vs_S-Line-C7H2-6h-EtOH.xls |
148.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_S-Line-PreB-24h-GC_vs_S-Line-PreB-6h-EtOH.xls |
520.5 Kb |
(ftp)(http) |
XLS |
| GSE2842_S-Line-PreB-6h-GC_vs_S-Line-PreB-6h-EtOH.xls |
80.5 Kb |
(ftp)(http) |
XLS |
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