GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE2843 Query DataSets for GSE2843
Status Public on Nov 14, 2005
Title thymic mouse cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Glucocorticoids (GC) are in most chemotherapy protocols for lymphoid malignancies, particularly childhood acute lymphoblastic leukaemia (ALL) for their ability to induce apoptosis in malignant blast. The underlying mechanism, however, has so far only been investigated in model systems. This study comprises Affymetrix hgu133 plus 2.0 analyses of
Peripheral blood lymphoblasts purified at three time points (0h, 6-8h, 24h after treatment initiation) from 13 children under therapy for ALL . Treated samples were compared to untreated (0h). For comparison, expression profiles were generated from an adult ALL patient, peripheral blood lymphocytes from GC-exposed healthy donors, GC-sensitive and -resistant ALL cell lines and mouse thymocytes treated with GC in vivo and in vitro. This series consists of the mouse thymocyte samples.
Findings. An essentially complete list of GC-regulated candidate genes in clinical settings experimental and was generated, enabling immediate analysis of any gene with respect to its potential significance for GC-induced apoptosis in these systems. Gene regulations previously thought responsible for cell death in experimental systems were reconfirmed in few children only. In contrast, a small number of genes, most not implicated in GC-induced apoptosis previously, were co-ordinately regulated in the majority of children.
Keywords: treatment response
Overall design In vivo and in vitro glucocorticoid treated samples were compared to their control samples (ethanol or PBS treated).
Contributor(s) Schmidt S, Rainer J, Riml S, Ploner C, Jesacher S, Achmüller C, Presul E, Skvortsov S, Crazzolara R, Fiegl M, Raivio T, Jänne OA, Geley S, Meister B, Kofler R
Citation(s) 16293608
Submission date Jun 28, 2005
Last update date Feb 11, 2019
Contact name Johannes Rainer
Organization name EURAC
Department Center for Biomedicing
Lab Biomedical Informatics
Street address Via Galvani 31
City Bolzano
ZIP/Postal code 39100
Country Italy
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (5)
GSM60604 Mouse-in vivo-4h-PBS
GSM60605 Mouse-in vivo-4h-GC
GSM60606 Mouse-in vitro-4h-GC
BioProject PRJNA92481

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE2843_E-Values-Mouse.xls 8.6 Mb (ftp)(http) XLS
GSE2843_M-Values-Mouse.xls 6.2 Mb (ftp)(http) XLS
GSE2843_Mouse-in vitro-4h-GC_vs_Mouse-in_vitro-4h-EtOH.xls 149.5 Kb (ftp)(http) XLS
GSE2843_Mouse-in_vivo-4h-GC_vs_Mouse-in_vivo-4h-PBS.xls 272.0 Kb (ftp)(http) XLS
GSE2843_RAW.tar 28.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap