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Status |
Public on Oct 05, 2011 |
Title |
RNA isolation from mouse skin biopies |
Organism |
Mus musculus |
Experiment type |
Other
|
Summary |
Study to optimize our protocol for isolating RNA from skin biopsies from (hairless) SKH mice using different-diameter biopsy punches. Some mice were also treated with UVB radiation to check its effect on RNA yield.
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Overall design |
4 mice total: 2 were irradiated with 300J/m2 UVB and 2 were non-irradiated. Post-mortem skin biopsies with 1.5mm, 2.0mm, and 2.5mm diameter punches were taken from the dorsal region.
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Contributor(s) |
Rodenburg W, de Vries A, de Jong HM, Bruning O, Breit TM |
Citation(s) |
22023775 |
Submission date |
Apr 07, 2011 |
Last update date |
Dec 06, 2013 |
Contact name |
Oskar Bruning |
Organization name |
University of Amsterdam
|
Department |
SILS
|
Lab |
MicroArray Department
|
Street address |
Science Park 904
|
City |
Amsterdam |
ZIP/Postal code |
1098 XH |
Country |
Netherlands |
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Platforms (1) |
GPL10192 |
NimbleGen Mus musculus MM9 Expression Array (12x135k) |
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Samples (12)
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Relations |
BioProject |
PRJNA139219 |