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Series GSE28470 Query DataSets for GSE28470
Status Public on Dec 01, 2011
Title NMLF response to spinal cord injury in Zebrafish
Organism Danio rerio
Experiment type Expression profiling by array
Summary The study was designed to identify genes regulated after spinal transection that might contribute to regenerative growth of neurons projecting from the NMLF in Zebrafish.
Zebrafish were injured by surgical transection of the spinal cord at 1 mm caudal to the brainstem-spinal cord junction (Injured). Animals receiving sham surgery (identical surgical procedures without transection) served as control (Control). The nucleus of the medial longitudinal fascicle (NMLF) was laser capture microdissected from approximately 30 frozen sections. RNA was prepared, amplified, and run on Affymetrix Zebrafish arrays.
Overall design Zebrafish were used because they recover swimming function after spinal transection in about 6 weeks. The NMLF has been identified as a prominent group of neurons that descend through the site of injury in the spinal cord and that regenerate after injury. Times were selected to distinguish early events from those in the timeframe of regenerative growth.
Contributor(s) Ma L, Hart RP, Schachner M
Citation(s) 22288476, 24747977
Submission date Apr 08, 2011
Last update date Aug 22, 2019
Contact name Ronald P. Hart
Phone 848-445-1783
Organization name Rutgers University
Department W.M. Keck Center for Collaborative Neuroscience
Street address 604 Allison Rd Rm D251
City Piscataway
State/province NJ
ZIP/Postal code 08854
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (18)
GSM703419 Zebrafish NMLF at 4h after surgery, sham control 1
GSM703420 Zebrafish NMLF at 4h after surgery, sham control 2
GSM703421 Zebrafish NMLF at 4h after surgery, sham control 3
BioProject PRJNA139209

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28470_RAW.tar 38.9 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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