NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE28505 Query DataSets for GSE28505
Status Public on Apr 13, 2011
Title Comparative global gene expression profiling of mice liver and spleen fed with nucleoprotein (NP) diets
Organism Mus musculus
Experiment type Expression profiling by array
Summary A diet rich in nucleic acids and protamin protein, termed as nucleoprotein was used for the study. Mice were fed with NP diets for 4 weeks followed by removal of the liver and spleen. Total RNA extracted from livers and spleens was pooled in each group (low NP or LNP-control, and 1.2% NP-treatment, diets), prior to DNA microarray analysis (Agilent mouse whole genome 4 x 44K). Results revealed 1373 & 3386 up (>1.5 fold)- and down (<0.75 fold)-regulated genes in the liver, and 252 & 1838 up- and down-regulated genes in the spleen, respectively following 1.2%NP diet. Analysis of genes related to NP diets will be discussed.
 
Overall design To investigate the effect of NP, we added S-nuclegen® at a concentration of 1.2% into CLEA basic purified diet (CLEA JAPAN, Inc., Tokyo, Japan) known to include nucleic acids (NAs) at much lower amounts than standard diet. By HPLC analysis CLEA basic purified diet (low NP), and 1.2% NP diet included 0.03%, and 0.5% NAs, respectively. Male C57BL/6J mice (13 weeks) were fed with NP diet for 4 weeks. The liver and spleen were removed and deep frozen in liquid nitrogen. Whole blood was also sampled from these mice, and frozen in liquid nitrogen. Total RNA extracted from livers and spleens was pooled in each group (control, lowNP or LNP; and treatment, 1.2%NP), prior to DNA microarray analysis (Agilent mouse whole genome 4 x 44K).
 
Contributor(s) Ogawa T, Saito T, Sawa C, Murayama A, Rakwal R, Shibato J, Shioda S
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 10, 2011
Last update date May 10, 2018
Contact name RANDEEP RAKWAL
E-mail(s) plantproteomics@gmail.com
Phone +81-(0)90-1853-7875
Organization name University of Tsukuba
Department Institute of Health and Sport Sciences
Lab GSI 403
Street address 1-1-1 Tennodai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8574
Country Japan
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (4)
GSM706173 LNP(Cy3)×1.2%NP L(Cy5)_Pooled Sample 1
GSM706174 LNP(Cy5)×1.2%NP L(Cy3)_Pooled Sample 2
GSM706175 LNP(Cy3)×1.2%NP S(Cy5)_Pooled Sample 1
Relations
BioProject PRJNA138983

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28505_RAW.tar 69.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap