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Status |
Public on Apr 13, 2011 |
Title |
Comparative global gene expression profiling of mice liver and spleen fed with nucleoprotein (NP) diets |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
A diet rich in nucleic acids and protamin protein, termed as nucleoprotein was used for the study. Mice were fed with NP diets for 4 weeks followed by removal of the liver and spleen. Total RNA extracted from livers and spleens was pooled in each group (low NP or LNP-control, and 1.2% NP-treatment, diets), prior to DNA microarray analysis (Agilent mouse whole genome 4 x 44K). Results revealed 1373 & 3386 up (>1.5 fold)- and down (<0.75 fold)-regulated genes in the liver, and 252 & 1838 up- and down-regulated genes in the spleen, respectively following 1.2%NP diet. Analysis of genes related to NP diets will be discussed.
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Overall design |
To investigate the effect of NP, we added S-nuclegen® at a concentration of 1.2% into CLEA basic purified diet (CLEA JAPAN, Inc., Tokyo, Japan) known to include nucleic acids (NAs) at much lower amounts than standard diet. By HPLC analysis CLEA basic purified diet (low NP), and 1.2% NP diet included 0.03%, and 0.5% NAs, respectively. Male C57BL/6J mice (13 weeks) were fed with NP diet for 4 weeks. The liver and spleen were removed and deep frozen in liquid nitrogen. Whole blood was also sampled from these mice, and frozen in liquid nitrogen. Total RNA extracted from livers and spleens was pooled in each group (control, lowNP or LNP; and treatment, 1.2%NP), prior to DNA microarray analysis (Agilent mouse whole genome 4 x 44K).
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Contributor(s) |
Ogawa T, Saito T, Sawa C, Murayama A, Rakwal R, Shibato J, Shioda S |
Citation missing |
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Submission date |
Apr 10, 2011 |
Last update date |
May 10, 2018 |
Contact name |
RANDEEP RAKWAL |
E-mail(s) |
plantproteomics@gmail.com
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Phone |
+81-(0)90-1853-7875
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Organization name |
University of Tsukuba
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Department |
Institute of Health and Sport Sciences
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Lab |
GSI 403
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Street address |
1-1-1 Tennodai
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City |
Tsukuba |
State/province |
Ibaraki |
ZIP/Postal code |
305-8574 |
Country |
Japan |
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Platforms (1) |
GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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Samples (4)
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GSM706173 |
LNP(Cy3)×1.2%NP L(Cy5)_Pooled Sample 1 |
GSM706174 |
LNP(Cy5)×1.2%NP L(Cy3)_Pooled Sample 2 |
GSM706175 |
LNP(Cy3)×1.2%NP S(Cy5)_Pooled Sample 1 |
GSM706176 |
LNP(Cy5)×1.2%NP S(Cy3)_Pooled Sample 2 |
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Relations |
BioProject |
PRJNA138983 |
Supplementary file |
Size |
Download |
File type/resource |
GSE28505_RAW.tar |
69.0 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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