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Series GSE28585 Query DataSets for GSE28585
Status Public on May 11, 2011
Title Dynamic Chromatin Localization of Sirt6 Shapes Stress- and Aging- Related Transcriptional Networks (Illumina)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Sirtuins (Sirt) are a family of enzymes that modify chromatin and other proteins to affect gene activity. Loss of Sirt6 leads to a progeria-like phenotype in mice, but the target of SIRT6 action has been elusive. Here we show that Sirt6 binds to thousands of gene promoters in a stress-inducible fashion, guided by the stress-responsive transcription factor NF-κB.
Chromatin profiling by ChIP-chip analysis of Sirt6 and NF-KB component RelA combined with expression array data of wildtype, Sirt6 knockout and Sirt6 RelA double knockout cells demonstrates that RelA recruits Sirt6 to NF-KB targets in response to TNF-a induction and that many of these targets are important for senescence and aging.
 
Overall design comparison of wild type, Sirt6-/- and Sirt6-/- RelA-/- MEF cells
 
Contributor(s) Tiara L K, Nicole A R, Angela R W, Kun Q, Stephan R Q, Howard Y C
Citation(s) 21738489
Submission date Apr 13, 2011
Last update date Jun 14, 2018
Contact name Kun Qu
E-mail(s) kqu@stanford.edu
Organization name Stanford University
Department Dermatology
Lab Howard Chang
Street address 269 Campus Dr. CCSR 2150
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM707632 WT_0min
GSM707633 WT_1.5 hrs
GSM707634 WT_6 hrs
This SubSeries is part of SuperSeries:
GSE28641 Dynamic Chromatin Localization of Sirt6 Shapes Stress- and Aging- Related Transcriptional Networks
Relations
BioProject PRJNA143009

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28585_RAW.tar 3.1 Mb (http)(custom) TAR
GSE28585_non-normalized.txt.gz 2.0 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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