GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE28585 Query DataSets for GSE28585
Status Public on May 11, 2011
Title Dynamic Chromatin Localization of Sirt6 Shapes Stress- and Aging- Related Transcriptional Networks (Illumina)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Sirtuins (Sirt) are a family of enzymes that modify chromatin and other proteins to affect gene activity. Loss of Sirt6 leads to a progeria-like phenotype in mice, but the target of SIRT6 action has been elusive. Here we show that Sirt6 binds to thousands of gene promoters in a stress-inducible fashion, guided by the stress-responsive transcription factor NF-κB.
Chromatin profiling by ChIP-chip analysis of Sirt6 and NF-KB component RelA combined with expression array data of wildtype, Sirt6 knockout and Sirt6 RelA double knockout cells demonstrates that RelA recruits Sirt6 to NF-KB targets in response to TNF-a induction and that many of these targets are important for senescence and aging.
Overall design comparison of wild type, Sirt6-/- and Sirt6-/- RelA-/- MEF cells
Contributor(s) Tiara L K, Nicole A R, Angela R W, Kun Q, Stephan R Q, Howard Y C
Citation(s) 21738489
Submission date Apr 13, 2011
Last update date Jun 14, 2018
Contact name Kun Qu
Organization name Stanford University
Department Dermatology
Lab Howard Chang
Street address 269 Campus Dr. CCSR 2150
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM707632 WT_0min
GSM707633 WT_1.5 hrs
GSM707634 WT_6 hrs
This SubSeries is part of SuperSeries:
GSE28641 Dynamic Chromatin Localization of Sirt6 Shapes Stress- and Aging- Related Transcriptional Networks
BioProject PRJNA143009

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28585_RAW.tar 3.1 Mb (http)(custom) TAR
GSE28585_non-normalized.txt.gz 2.0 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap