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Series GSE28621 Query DataSets for GSE28621
Status Public on May 09, 2014
Title Transcriptional profiles of macrophages in resolving inflammation
Organism Mus musculus
Experiment type Expression profiling by array
Summary We have performed a comprehensive transcriptional analysis of specific monocyte and macrophage (MØ) subsets during an acute self-resolving inflammatory insult. Following initial induction of acute inflammation, tissue resident (Resident) MØ are rapidly ‘cleared’ from the inflammatory foci, only becoming recoverable as inflammation resolves. Monocytes are recruited to the inflammatory lesion where they differentiate into MØ. We term these monocyte-derived MØ ‘inflammation-associated’ to distinguish them from Resident MØ which are present throughout the inflammatory response and can renew during the resolution of inflammation by proliferation. Comparative analysis of the Mo and MØ populations (both ‘inflammation-associated’ and Resident MØ) identifies select genes expressed in subsets of ‘inflammation-associated’ and Resident MØ that play important roles in the resolution of inflammation and/or for immunity, including molecules involved in antigen presentation, cell cycle and others associated with ‘immaturity’ and MØ activation.
 
Overall design We purified monocyte and macrophage populations from the peritoneal cavity of C57BL/6 mice 4, 18, 72 and 168 hours after the induction of inflammation with intraperitoneal administration of zymosan (2x10^7 particles). We also purified tissue resident macrophages and Ly-6B+ bone marrow monocytes from naive mice as reference populations.
 
Contributor(s) Taylor PR, Rosas M, Giles PJ
Citation(s) 24762537
Submission date Apr 14, 2011
Last update date Feb 11, 2019
Contact name Peter Giles
E-mail(s) GilesPJ@Cardiff.ac.uk
Organization name Cardiff University
Street address Heath Park
City CARDIFF
ZIP/Postal code CF14 4XN
Country United Kingdom
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (21)
GSM709379 F4/80(med-high), Ly-6B+ inflammation-associated macrophages_168 hours_biological rep 1
GSM709380 F4/80(med-high), Ly-6B+ inflammation-associated macrophages_168 hours_biological rep 2
GSM709381 F4/80(med-high), Ly-6B+ inflammation-associated macrophages_168 hours_biological rep 3
Relations
BioProject PRJNA138723

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28621_RAW.tar 89.4 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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