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| Status |
Public on Apr 15, 2011 |
| Title |
Comprehensive gene expression analysis of human embryonic stem cell during differentiation into neural cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In this research we have done a comprehensive transcripteome analysis of hESC differentiation at three different stages: early neural differentiation, neural ectoderm, and mature neurons. We detected and validated time-dependent gene expression patterns and represented that the gene expression patterns exhibit early ESC differentiation. Pathway analysis revealed dynamic expression patterns of members of several signaling pathways.
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| Overall design |
The hESC cells were passaged every 7 days using collagenase/dispase and cultured under feeder-free culture conditions on Matrigel in hESC medium. Three biological replicates per time point served as the input to generate biotin-labeled cRNA using a linear amplification kit.
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| Contributor(s) |
Fathi A, Hatami M, Hajihoseini V, Fattahi F, Baharvand H, Salekdeh GH |
| Citation(s) |
21829537 |
| Submission date |
Apr 14, 2011 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Vahid Hajihoseini |
| E-mail(s) |
vdhoseini@gmail.com
|
| Phone |
+98-21-22305236
|
| Fax |
+98-21-22306481
|
| URL |
http://www.royaninstitute.org
|
| Organization name |
Royan Institute
|
| Department |
Systems Biology
|
| Lab |
Bioinformatics
|
| Street address |
Banihashem st, resalat ave
|
| City |
Tehran |
| State/province |
Tehran |
| ZIP/Postal code |
98 |
| Country |
Iran |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (12)
|
| GSM709608 |
Human embryonic stem cell - replicate 1 |
| GSM709609 |
Human embryonic stem cell - replicate 2 |
| GSM709610 |
Human embryonic stem cell - replicate 3 |
| GSM709611 |
Neural initiation stage of human embryonic stem cell-replicate 1 |
| GSM709612 |
Neural initiation stage of human embryonic stem cell-replicate 2 |
| GSM709613 |
Neural initiation stage of human embryonic stem cell-replicate 3 |
| GSM709614 |
Neural ectoderm Derived from human embryonic stem cell-replicate 1 |
| GSM709615 |
Neural ectoderm Derived from human embryonic stem cell-replicate 2 |
| GSM709616 |
Neural ectoderm Derived from human embryonic stem cell-replicate 3 |
| GSM709617 |
Mature Neural Cells Derived from hESC-replicate 1 |
| GSM709618 |
Mature Neural Cells Derived from hESC-replicate 2 |
| GSM709619 |
Mature Neural Cells Derived from hESC-replicate 3 |
|
| Relations |
| BioProject |
PRJNA138883 |
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