 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 19, 2011 |
| Title |
Peripheral blood RNA expression profiling in illicit methcathinone users reveals effect on immune system |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Methcathinone (ephedrone) is relatively easily accessible for abuse. Its users develop an extrapyramidal syndrome and it is not known if this is caused by methcathinone itself, by side-ingredients (manganese), or both. In the present study we aimed to clarify molecular mechanisms underlying this condition. We used microarrays to analyze whole genome gene expression patterns of peripheral blood from 20 methcathinone users and 20 matched controls. Gene expression profile data were analyzed by Bayesian modelling and functional annotation. Of 28,869 genes on the microarrays, 326 showed statistically significant differential expression with FDR adjusted p-values below 0.05. Quantitative RT-PCR confirmed differential expression for the most of the genes selected for validation. Functional annotation and network analysis indicated activation of a gene network that included immunological disease, cellular movement and cardiovascular disease functions (enrichment score 42). As HIV and HCV infections were confounding factors, we performed additional stratification of patients. A similar functional activation of the “immunological disease” category was evident when we compared patients according to injection status (past versus current users, balanced for HIV and HCV infection). However, this difference was not large therefore the major effect was related to the HIV status of the patients. Mn-methcathinone abusers have blood RNA expression patterns that mostly reflect their HIV and HCV infections. However, despite the strong confounding effect from infection, some modest drug abuse effects on gene expression were detected.
|
| |
|
| Overall design |
40 samples, 20 healthy volunteers and 20 illicit methcathinone users
|
| |
|
| Contributor(s) |
Sikk K, Kõks S, Soomets U, Schalkwyk LC, Fernandes C, Haldre S, Aquilonius S, Taba P |
| Citation(s) |
22303338 |
|
Sikk K, Kõ, Schalkwyk LC, Fernandes C, Haldre S, Aquilonius S-M and Taba P (2011) Peripheral blood RNA expression profiling in illicit methcathinone users reveals effect on immune system. Front. Gene. 2:42. doi: 10.3389/fgene.2011.00042 http://www.frontiersin.org/neurogenomics/10.3389/fgene.2011.00042/abstract
|
| Submission date |
Apr 18, 2011 |
| Last update date |
Sep 24, 2019 |
| Contact name |
Sulev Koks |
| E-mail(s) |
sulev.koks@murdoch.edu.au
|
| Phone |
+61864570313
|
| Organization name |
Murdoch University
|
| Department |
Centre for Molecular Medicine and Innovative Therapeutics
|
| Street address |
90 South Street
|
| City |
Perth |
| State/province |
Western Australia |
| ZIP/Postal code |
6150 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (40)
|
|
| Relations |
| BioProject |
PRJNA138763 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28686_RAW.tar |
171.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...