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| Status |
Public on Jul 26, 2011 |
| Title |
Transgenic overexpression of Tcfap2c/AP-2gamma results in liver steatosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We established a mouse model, in which transcription factor Tcfap2c can be activated in an inducible and reversible manner in somatic tissues, taking advantage of the tetracycline-dependent regulatory system.
Induction of the transgene led to robust expression in all tissues (except brain and testis) and lead to rapid mortality within 3-7 days. In the liver, accumulation of microvesicular lipid droplets and breakdown of major hepatic metabolism pathways resulted in steatosis.
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| Overall design |
Transgenic and control mice were treated with doxycycline (1mg/d i.p.) three times (0, 24 and 48h) and liver samples were collected after 52h. In addition, primary hepatocytes isolated from transgenic and control animals were cultured with doxycycline for 3 days (1µg/ml).
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| Contributor(s) |
Kuckenberg P, Schorle H |
| Citation(s) |
21779369 |
| Submission date |
Apr 18, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Peter Kuckenberg |
| URL |
http://www.meb.uni-bonn.de/devpatho/
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| Organization name |
University Medical School Bonn
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| Department |
Institut of Pathology
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| Lab |
Prof. Schorle
|
| Street address |
Sigmund Freud Str. 25
|
| City |
Bonn |
| ZIP/Postal code |
53127 |
| Country |
Germany |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA138903 |
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