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| Status |
Public on Apr 19, 2011 |
| Title |
Development of gene expression signatures on immunity for bursal peptide BPP-II |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To further elucidate the mechanism of BPP-II on immunity on the broad molecular level, we have employed whole genome microarray expression profiling as a discovery platform to examine gene expression patterns during BPP-II treatment in a mouse derived hybridoma culture system. Hybridoma cell was treated ex vivo, and robust normalization of the data identified 2478 differentially expressed probe sets exhibiting minimum 1.5-fold changes that distinguished between BPP-II and control samples. Various pathwayswere significantly impacted by BPP-II treatment, and gene Ontology annotations show changes in the expression of molecules involved in immune and immunocyte related cellular processes. Expression of 8 genes (MS4A2，CD86，CD80， Fas，CDKN2A，CBLC, FGF21 and Fyn) from this signature was quantified in the RNA samples by QRT-PCR, confirming low variability between the predicted response patterns.
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| Overall design |
BPP-II induced gene expression in hybridoma cell was measured at 4 hours after exposure to doses of 0ug/ml and 0.2ug/ml. Two independent experiments were performed using different cells for each experiment.
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| Contributor(s) |
Chen PY, Feng XL |
| Citation missing |
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| Submission date |
Apr 18, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Pu Yan Chen |
| E-mail(s) |
puyanchen2010@yahoo.com.cn
|
| Organization name |
Nanjing Agriculture University
|
| Street address |
No. 1, Weigang
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| City |
Nanjing |
| ZIP/Postal code |
210095 |
| Country |
China |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (2) |
| GSM675882 |
ExVivoHybridomacell_4hr_0ug/ml_rep1 |
| GSM710704 |
ExVivoHybridomacell_4hr_0.2ug/ml_rep1 |
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| Relations |
| BioProject |
PRJNA138907 |
