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Series GSE28735 Query DataSets for GSE28735
Status Public on Jul 09, 2012
Title Microarray gene-expression profiles of 45 matching pairs of pancreatic tumor and adjacent non-tumor tissues from 45 patients with pancreatic ductal adenocarcinoma
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In order to identify biologically relevant tumor markers with prognostic significance, we set out to analyze gene expression profiling of tumor and adjacent non-tumor tissues from PDAC cases.
We compared the microarray gene-expression profiles of 45 matching pairs of pancreatic tumor and adjacent non-tumor tissues. This data set were used to obtained genes that were differentially expressed and associated with survival. 51 genes were selected for further validation.
 
Overall design 90 total samples were analyzed. We compared gene expression profile of 45 pairs of pancreatic tumor and adjacent non-tumor tissues using Affymetrix GeneChip Human Gene 1.0 ST arrays. Tumor gene expression profiles were distinctly different from non-tumor profiles. Using ANOVA in PartekĀ®, 7352 independent genes were found to be differentially expressed in tumors (P<0.01). We next performed Cox-regression analysis and identified 928 of the differentially expressed genes that were associated with survival (P<0.1). The list of genes was then subjected to pathway and biomarker analyses using Ingenuity Pathways Analysis (IPA). Based on literatures supporting their roles in cancer, 51 genes were selected for further analyses
 
Contributor(s) Hussain SP
Citation(s) 22363658, 23918603
Submission date Apr 20, 2011
Last update date Jul 26, 2018
Contact name Perwez Hussain
E-mail(s) hussainp@mail.nih.gov
Organization name NCI/NIH
Street address 37 Convent Drive
City Bethesda
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (90)
GSM711904 human pancreatic tumor tissue, patient sample 1
GSM711905 human pancreatic nontumor tissue, patient sample 1
GSM711906 human pancreatic tumor tissue, patient sample 2
Relations
BioProject PRJNA140549

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Supplementary file Size Download File type/resource
GSE28735_RAW.tar 314.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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