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| Status |
Public on Dec 31, 2014 |
| Title |
CD24-low Cancer stem-cell like cell fraction in Notch inhibited transformed human esophageal cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Comparison of CD24-low/CD44-high (CD44H) and CD24-high/CD44-low (CD44L) cell fractions in transformed human esophageal cells with Notch inhibition. CD44H cells display enhanced malignant potential (e.g. tumorigenicity, colony formation) compared to CD44L cells.
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| Overall design |
NOTCH signaling was suppressed by retrovirus-mediated stable transduction of dominant-negative Mastermind-like1 (DNMAML1) in transformed human esophageal keratinocyte cell line EPC2-T (EPC2-hTERT-EGFR-cyclin D1-p53R175H) as described (Gastroenterology. 2010;139:2113-23), resulting in creation of cell line designated 4801. 4801 cells were sorted by flow cytometry for a cell fraction expressing a low level of CD24 and a high level of CD44 (CD44H) as well as that expressing a high level of CD24 and a low level of CD44 (CD44L). The former (CD44H) displayed fibroblast-like mesenchymal morphology while the latter (CD44L) remained cuboidal epithelioid morphology in culture. CD24-low and CD24-high cells were subjected to RNA extraction and gene array experiments.
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| Contributor(s) |
Nakagawa H, Rustgi AK |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 22, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Hiroshi Nakagawa |
| E-mail(s) |
nakagawh@mail.med.upenn.edu
|
| Phone |
215-573-1867
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| Organization name |
University of Pennsylvania
|
| Department |
Gastroenterology Division
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| Lab |
Nakagawa
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| Street address |
415 Curie Blvd.
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM713322 |
CD24-low/CD44-high (CD44H) cell fraction 1 |
| GSM713324 |
CD24-low/CD44-high (CD44H) cell fraction 2 |
| GSM713326 |
CD24-low/CD44-high (CD44H) cell fraction 3 |
| GSM713327 |
CD24-high/CD44-low (CD44L) cell fraction 1 |
| GSM713330 |
CD24-high/CD44-low (CD44L) cell fraction 2 |
| GSM713331 |
CD24-high/CD44-low (CD44L) cell fraction 3 |
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| Relations |
| BioProject |
PRJNA138823 |
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