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Series GSE28811 Query DataSets for GSE28811
Status Public on Oct 04, 2011
Title Reprogramming is achieved within a single cell cycle after mouse nuclear transfer
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although nuclear transfer allows the reprogramming of somatic cells to totipotency, little is known concerning the kinetics by which it takes place or the minimum requirements for its success. Here, we demonstrate that reprogramming can be achieved within a few hours and a single cell-cycle as long as two key constraints on reprogramming are satisfied. First, the recipient cell chromosomes must be removed during mitosis. Second, the nuclear envelope of the donor cell must be broken down and its chromosomes condensed, allowing an embryonic nucleus to be constructed around the incoming chromosomes. If these requirements are not met, then reprogramming fails and embryonic development arrests. These results point to a central role for processes intimately linked to cell division in mediating efficient transitions between transcriptional programs.
Overall design tail tip skin fibroblasts were transferred into mitotic mouse zygotes, blastomeres or oocytes.
Contributor(s) Egli D, Eggan K
Citation(s) 21971503
Submission date Apr 22, 2011
Last update date Jun 14, 2018
Contact name Dieter Egli
Phone 617 4968659
Organization name The New York Stem Cell Foundation Research Institute
Department NYSCF
Lab Egli
Street address 3960 Broadway
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (2)
GPL6103 Illumina mouseRef-8 v1.1 expression beadchip
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (37)
GSM713430 cleavage inhibition control (4991825016 B)
GSM713431 2-cell stage control embryo (4991825016 C)
GSM713432 2-cell stage control embryo (4991825016 D)
BioProject PRJNA138795

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28811_RAW.tar 6.5 Mb (http)(custom) TAR
GSE28811_non-normalized_GPL6103.txt.gz 3.4 Mb (ftp)(http) TXT
GSE28811_non-normalized_GPL6885.txt.gz 2.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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