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Series GSE28840 Query DataSets for GSE28840
Status Public on Apr 25, 2011
Title Glucocorticoid receptor (GR) target genes in C2C12 myotubes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Analysis of C2C12 myotubes treated with dexamethasone (Dex) for 6 or 24 hours. Dex is a synthetic glucorticoid receptor agonist. Results provide insight to the effect of glucocorticoids on myotubes.
 
Overall design C2C12 myotubes were cultured in DMEM supplemented with 2% horse serum, and treated with 1 uΜ Dex or an equal volume (0.01% v/v of media) of vehicle control ethanol for 6 or 24 hours. Total cellular RNA was isolated utilizing the NucleoSpin RNA II kit (Macherey-Nagel). RNA isolates were first quantified by standard spectrophotometry, and then qualitatively evaluated by capillary electrophoresis employing the Bio-Rad Experion system per manufacturer’s instruction. The final labeled cRNA samples were hybridized overnight to Illumina mouseWG-6 BeadChip arrays, which was performed at UCSF Genomic Core. All treatments were done in triplicates and the same batch of microarrays were used for all treatments. The Illumina expression arrays were pre-processed using lumi package. The differential expression analysis was performed using the Limma package.
 
Citation(s) 22733784
Submission date Apr 25, 2011
Last update date Jan 16, 2019
Contact name Jen-Chywan Wang
E-mail(s) walwang@berkeley.edu
Phone 510-643-1039
Organization name UC Berkeley
Department Nutritional Sciences & Toxicology
Street address 315 Morgan Hall, UC Berkeley
City Berkeley
State/province CA
ZIP/Postal code 94720-3104
Country USA
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (18)
GSM714189 myotube_ETOH_6h_rep1
GSM714190 myotube_ETOH_6h_rep2
GSM714191 myotube_ETOH_6h_rep3
Relations
BioProject PRJNA140617

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28840_RAW.tar 15.8 Mb (http)(custom) TAR
Processed data included within Sample table

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