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| Status |
Public on Sep 13, 2012 |
| Title |
RNA-seq experiments in human neural crest cells (hNCC) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Combining an in vitro hNCC differentiation protocol with epigenomic profiling, we provide the first whole-genome characterization of cis-regulatory elements in this highly relevant cell type. With this data at hand, we have characterized the chromatin state and dynamics of all human gene promoters during the course of NCC in vitro differentiation. Most importantly, we have identified a large cohort of active and NCC-specific enhancers, which we showed to be functionally relevant in vivo, in the context of embryonic development. Finally, through sequence analysis of the identified NCC enhancers, we uncovered the orphan nuclear receptors NR2F1 and NR2F2 as novel hNCC transcriptional regulators both in vitro and in vivo.
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| Overall design |
RNA-seq experiments in human neural crest cells (hNCC)
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| Contributor(s) |
Rada-Iglesias A, Wysocka J |
| Citation(s) |
22981823 |
| Submission date |
Apr 27, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Alvaro Rada |
| E-mail(s) |
alvaror@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Chemical and Systems Biology
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| Lab |
Wysocka lab
|
| Street address |
269 Campus Drive, CCSR Building
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE28876 |
Epigenomic profiling of enhancers identifies orphan nuclear receptors NR2F1 and NR2F2 as novel regulators of human neural crest |
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| Relations |
| SRA |
SRP006561 |
| BioProject |
PRJNA153965 |
