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| Status |
Public on Apr 27, 2011 |
| Title |
Differentially expressed genes in metallopanstimulin-1 knockdown cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To identify the differentially expressed genes in metallopanstimulin-1 (MPS-1) knockdown gastric cancer cells compared with negative control ones, we employed the microarray profiling analysis. MPS-1 was knockdown by retroviral interfering system in human gastric adenocarcinoma originated cell SGC7901 and the transfectants named P4, while the negative control named NC. Genes with greater than 1.5-fold change and P-value ＜0.05 were identified as differentially expressed genes between NC and P4 cells. Among those, apoptotic related gene (Gadd45β, cIAP2, Bcl2, CAD, Bid, etc) and adhesive related genes (integrin beta 4, ECM2, etc) were quantified by real-time PCR as well as western blotting.
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| Overall design |
The two groups of negative control (NC) and MPS-1 knockdown gastric cancer cells (P4) were harvested after puromycin screening. Three independent experiments were performed for each group.
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| Contributor(s) |
Yang Z, Gu Q |
| Citation(s) |
21796632 |
| Submission date |
Apr 27, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Zhongyin Yang |
| E-mail(s) |
jeffreyyong@163.com
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| Organization name |
Ruijin Hospital, Shanghai Jiao Tong University School of Medicine
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| Department |
Department of Surgery
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| Street address |
NO.197 Ruijin er road
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| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (6)
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| GSM714815 |
Gastric cancer cells_negative control_1_rep1 |
| GSM714816 |
Gastric cancer cells_negative control_1_rep2 |
| GSM714817 |
Gastric cancer cells_negative control_1_rep3 |
| GSM714818 |
Gastric cancer cell, MPS-1 knockdown, rep1 |
| GSM714819 |
Gastric cancer cell, MPS-1 knockdown, rep2 |
| GSM714820 |
Gastric cancer cell, MPS-1 knockdown, rep3 |
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| Relations |
| BioProject |
PRJNA140599 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28877_RAW.tar |
61.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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