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| Status |
Public on Jan 24, 2012 |
| Title |
MicroRNA expression data from human breast cancer cell lines after demethylation treatment. |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The contribution of aberrant DNA methylation and the downstream effects in tumorogenesis through silencing of tumor suppressor genes (TSGs) and microRNAs has been investigated. Since these epigenetic alterations can be reversed, we investigated the effects of the epigenetic therapy in breast cancer cell lines.
We used microarrays to investigate the global microRNA expression profile after demethylation treatment with 5-aza-2’-deoxycytidine (DAC) in breast cancer cell lines and identified distinct classes of early and late systematic stable or transient effects of the treatment.
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| Overall design |
Three selected breast cell lines including MDA-MB231, SKBR3, BT549, HS578T, MCF7 and HB2 (a breast epithelial cell line as control) were subject for miRNA isolation before treatment, after treatment with DAC and at five point follow-ups (1st, 3rd, 5th, 7th and 10th passages) at “drug holiday” condition and hybridized on Affymetrix microarrays.
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| Contributor(s) |
Radpour R, Staedtler F, Schumacher MM, Hartmann N |
| Citation(s) |
22076154 |
| Submission date |
Apr 29, 2011 |
| Last update date |
May 02, 2017 |
| Contact name |
Ramin Radpour |
| Organization name |
University of Bern
|
| Department |
Department for BioMedical Research
|
| Street address |
Murtenstrasse 35
|
| City |
Bern |
| ZIP/Postal code |
3008 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
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| Samples (27)
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| This SubSeries is part of SuperSeries: |
| GSE28976 |
Expression data from human breast cancer cell lines after demethylation treatment |
|
| Relations |
| BioProject |
PRJNA142859 |
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