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Series GSE29005 Query DataSets for GSE29005
Status Public on May 03, 2011
Title Gene expression microarray for mouse ES cells vs. mouse ES cells treated with 48 hr N2B27
Organism Mus musculus
Experiment type Expression profiling by array
Summary Core circuits of transcription factors stabilize stem and progenitor cells by suppressing genes required for differentiation. We do not know how such core circuits are reorganized during cell fate transitions to allow differentiation and lineage choice to proceed. Here, we asked how the pluripotency circuit, a core transcriptional circuit that maintains mouse embryonic stem (ES) cells in a pluripotent state, is dismantled as ES cells differentiate and choose between the neural ectodermal and mesendodermal progenitor cell fates. When ES cells are recultured from pluripotency maintaining conditions to the basal media N2B27, the expression of the pluripotency circuit genes begins to change. At 48 hours post N2B27 addition, the ES cells are competent to respond to differentiation signals. Here, our microarray analysis compares the gene expression profile of ES cells vs. the gene expression profile of cells that have been treated with N2B27 for 48 hours, reaching the competent state.
 
Overall design 2 x mouse ES cells in pluripotency maintaining conditions. 3 x mouse ES cells after 48 hr of N2B27 culture
 
Contributor(s) Thomson M, Liu SJ, Ramanathan S
Citation(s) 21663792
Submission date May 02, 2011
Last update date Jun 14, 2018
Contact name Matt Thomson
E-mail(s) thomson@post.harvard.edu
Organization name Harvard University
Department MCB
Lab Ramanathan
Street address 52 Oxford Street 368
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (5)
GSM718702 ESC Rep 1
GSM718703 ESC Rep 2
GSM718704 ESC N2B27 48 hr Rep 1
Relations
BioProject PRJNA140635

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29005_RAW.tar 3.1 Mb (http)(custom) TAR
GSE29005_non-normalized.txt.gz 910.3 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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