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| Status |
Public on May 07, 2011 |
| Title |
Prospective isolation of a bipotential clonogenic liver progenitor cell in adult mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The molecular identification of adult hepatic stem/progenitor cells has been hampered by the lack of truly specific markers. To isolate putative adult liver progenitor cells, we used cell surface-marking antibodies, including MIC1-1C3, to isolate subpopulations of liver cells from normal adult mice or those undergoing an oval cell response and tested their capacity to form bilineage colonies in vitro. Robust clonogenic activity was found to be restricted to a subset of biliary duct cells antigenically defined as CD45–/CD11b–/CD31–/MIC1-1C3+/CD133+/CD26–, at a frequency of one of 34 or one of 25 in normal or oval cell injury livers, respectively. Gene expression analyses revealed that Sox9 was expressed exclusively in this subpopulation of normal liver cells and was highly enriched relative to other cell fractions in injured livers. In vivo lineage tracing using Sox9creERT2-R26RYFP mice revealed that the cells that proliferate during progenitor-driven liver regeneration are progeny of Sox9-expressing precursors. A comprehensive array-based comparison of gene expression in progenitor-enriched and progenitor-depleted cells from both normal and DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine or diethyl1,4-dihydro-2,4,6-trimethyl- 3,5-pyridinedicarboxylate)-treated livers revealed new potential regulators of liver progenitors.
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| Overall design |
Treated and untreated cells were sorted by cell surface markers then compared.
M+/133+ untreated: 4 biological and 1 technical replicate - total of 5
M-/133- untreated: 2 biological and 1 technical replicate - total of 3
M+/133+ DDC-treated: 4 biological replicates - total of 4
M-/133- DDC-treated: 4 biological replicates - total of 4
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| Contributor(s) |
Dorrell C, Erker L, Schug J, Kopp JL, Canaday PS, Fox AJ, Smirnova O, Duncan AW, Finegold MJ, Sande M, Kaestner KH, Grompe M |
| Citation(s) |
21632826 |
| Submission date |
May 06, 2011 |
| Last update date |
May 10, 2018 |
| Contact name |
Jonathan Schug |
| E-mail(s) |
jschug@pennmedicine.upenn.edu
|
| Phone |
215 898 0773
|
| Organization name |
University of Pennsylvania
|
| Department |
School of Medicine
|
| Lab |
Next-Generation Sequencing Core
|
| Street address |
12-156 Smilow Ctr Trans Research
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104-5160 |
| Country |
USA |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA140387 |
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