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Series GSE29150 Query DataSets for GSE29150
Status Public on Nov 29, 2011
Title NSD2 links dimethylation of histone H3 at lysine 36 to oncogenic programming [Transduction]
Organism Mus musculus
Experiment type Expression profiling by array
Summary NSD2 (also named MMSET and WHSC1) is a histone lysine methyltransferase that is implicated in diverse diseases and commonly overexpressed in multiple myeloma due to a recurrent t(4;14) chromosomal translocation. However, the precise catalytic activity of NSD2 is obscure, preventing progress in understanding how this enzyme influences chromatin biology and myeloma pathogenesis. Here we show that dimethylation of histone H3 at lysine 36 (H3K36me2) is the principal chromatin-regulatory activity of NSD2. Catalysis of H3K36me2 by NSD2 is sufficient for gene activation. In t(4;14)-positive myeloma cells, the normal genome-wide and gene-specific distribution of H3K36me2 is obliterated, creating a chromatin landscape that selects for a transcription profile favorable for myelomagenesis. Catalytically active NSD2 confers xenograft tumor formation and invasion capacity upon t(4;14)-negative cells and NSD2 promotes oncogenic transformation of primary cells in an H3K36me2-dependent manner. Together our findings establish H3K36me2 as the primary product generated by NSD2, and demonstrate that genomic disorganization of this canonical chromatin mark initiates oncogenic programming.
 
Overall design Genome-wide expression profiling of p19ARF-/- mouse embryonic fibroblasts stably transduced with control vector or wild-type NSD2. Each cell line is tested in triplicate.
 
Contributor(s) Kuo AJ, Cheung P, Chen K, Zee BM, Lauring J, Xi Y, Park BH, Shi X, Kioi M, Garcia BA, Li W, Gozani O
Citation(s) 22099308
Submission date May 09, 2011
Last update date Jun 14, 2018
Contact name kaifu chen
E-mail(s) kaifuc@bcm.edu
Organization name Baylor College of Medicine
Street address 1 Baylor plaza, suite 450A
City Houston
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM721552 Trans_control_rep1
GSM721553 Trans_control_rep2
GSM721554 Trans_NSD2_rep1
This SubSeries is part of SuperSeries:
GSE29305 NSD2 links dimethylation of histone H3 at lysine 36 to oncogenic programming
Relations
BioProject PRJNA142881

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29150_RAW.tar 3.1 Mb (http)(custom) TAR
GSE29150_non-normalized_data.txt.gz 521.0 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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