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Series GSE2917 Query DataSets for GSE2917
Status Public on Dec 21, 2005
Title Profiling of visceral sensory neurons in dorsal root and nodose ganglia in BALB/c mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Vagal afferent neurons are thought to convey primarily physiological information, whereas spinal afferents transmit noxious signals from the viscera to the central nervous system. In order to elucidate molecular identities for these different properties, we compared gene expression profiles of neurons located in nodose ganglia (NG) and dorsal root ganglia (DRG) in mice. Intraperitoneal administration of Alexa Fluor-488 conjugated Cholera toxin B allowed identification of neurons projecting to the viscera. Fluorescent neurons in DRG (from T10 to T13) and NG were isolated using laser capture microdissection. Gene expression profiles of visceral afferent neurons, obtained by microarray hybridization, were analysed using multivariate spectral map analysis, SAM algorithm (Significance Analysis of Microarray data) and fold-difference filtering. A total of 1996 genes were found to be differentially expressed in DRG versus NG, including 41 G-protein coupled receptors and 60 ion channels. Expression profiles obtained on laser-captured neurons were contrasted to those obtained on whole ganglia demonstrating striking differences and the need for microdissection when studying visceral sensory neurons because of dilution of the signal by somatic sensory neurons. Furthermore, a detailed catalogue of all adrenergic and cholinergic, GABA, glutamate, serotonin and dopamine receptors, voltage-gated potassium, sodium and calcium channels and transient receptor potential cation channels present in visceral afferents is provided. Our genome-wide expression profiling data provide novel insight into molecular signatures that underlie both functional differences and similarities between NG and DRG visceral sensory neurons. Moreover, these findings will offer novel insight into mode of action of pharmacologic agents modulating visceral sensation.
Keywords: Cell type comparison
Overall design Three separate experiments were performed. First, 5 whole dorsal root ganglia were compared to 7 whole nodose ganglia. Second, Laser captured visceral neurons derived from 5 dorsal root ganglia and 5 nodose ganglia were compared on MG-U74Av2. Third, Laser captured visceral neurons derived from 9 dorsal root ganglia and 11 nodose ganglia were compared on Mouse430_2.
Contributor(s) Peeters PJ, Aerssens J, de Hoogt R, Stanisz A, Göhlmann HW, Hillsley K, Meulemans A, Grundy D, Stead RH, Coulie B
Citation(s) 16303873
Submission date Jul 11, 2005
Last update date Feb 11, 2019
Contact name Pieter Johan Peeters
Organization name J&J Pharmaceutical R&D
Department Internal Medicine
Street address Turnhoutseweg 30
City Beerse
ZIP/Postal code 2340
Country Belgium
Platforms (2)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (42)
GSM63653 DRG070_a
GSM63654 DRG084_a
GSM63655 DRG019_a
BioProject PRJNA92603

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