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| Status |
Public on Mar 01, 2012 |
| Title |
Gene expression changes upon Dot1L knockdown and Dot1L inhibitor treatment during reprogramming |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Through a loss-of-function approach, we identified that inhibition of the histone methyltransferase, Dot1L, accelerated somatic cell reprogramming, significantly increased the yield of induced pluripotent stem (iPS) cell colonies, and substituted for Klf4 and c-Myc in the reprogramming cocktail. To understand the mechanism by which Dot1L inhibition results in these phenotypes, we carried out gene expression profiling using Affymetrix microarrays.
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| Overall design |
GSM723207-GSM723224: Embryonic stem cell-derived fibroblasts (dH1fs) were retrovirally transduced in culture with vectors expressing either a control shRNA or an shRNA targeting Dot1L. These cells were then superinfected with either Oct4, Sox2, Klf4 and c-Myc (OSKM) retroviruses or Oct4, Sox2 and c-Myc (OSM) retroviruses. Total RNA was harvested 6 days later. There were three biologic replicates for each condition.
GSM880675-GSM880682: dH1fs were treated with 10uM Dot1L inhibitor (EPZ004777) and then superinfected with Oct4, Sox2, Klf4 and c-Myc (OSKM) retroviruses. Total RNA was harvested 6 days later. There were two biologic replicates for each condition.
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| Contributor(s) |
Onder TT, Daley GQ |
| Citation(s) |
22388813 |
| Submission date |
May 12, 2011 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Tamer T Onder |
| Organization name |
Children's Hospital Boston
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| Street address |
One Blackfan Circle
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (2) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
| GPL3921 |
[HT_HG-U133A] Affymetrix HT Human Genome U133A Array |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA139971 |
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